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Article 7(a)
1. Blood samples shall be taken, by or under the supervision of an officer of the Minister, from domestic fowls between 3 and 5 weeks before they are transferred to laying accommodation or when they are between 16 and 22 weeks of age, whichever occurs later.
2. The number of birds from which blood samples shall be taken shall be as follows–
(a)in the case of birds at grandparent level and above, all birds; and
(b)in the case of birds at parent level, a number calculated in accordance with the following table–
Number of birds at parent level required to be sampled for testing for Salmonella pullorum
Number of birds kept in a house or, on premises on which birds have free access to more than one house, number of birds in each group of houses on such premises | Number of birds to be sampled in that house or in that group of houses on those premises |
---|---|
300 or less | All birds up to 150 |
301–400 | 160 |
401–4000 | 170 |
4001 or more | 180 |
3. The samples shall comprise 0.02 ml of blood taken from a wing vein of a bird by pricking with a suitable needle.
Article 7(b)
The rapid plate whole blood test shall be used for the testing of blood samples forSalmonella pullorum which tests shall be carried out as follows–
(1) 0.02 ml of blood taken from a wing vein of a bird, after pricking with a suitable needle, shall be placed on a white ceramic tile using a loop of the appropriate size.
(2) 0.04 ml of polyvalent crystal violet stainedSalmonella pullorum antigen(1) shall be added to the blood and mixed with it.
(3) The tile shall be rocked gently for 2 minutes after which time the test shall be read.
(4) All bleeding needles and loops must be washed in a normal saline solution(2) after each bird has been sampled and tested which solution must be renewed after every 200 birds have been sampled and tested.
S. pullorum antigen must contain standard and variant strains ofS. pullorum stained with crystal violet and standardised against international standard sera raised against the standard and variant strains (O.I.E. 1986).
Normal saline solution is prepared by dissolving saline tablets in water according to the manufacturers instructions. Reference: O.I.E. (1986) International Zoosanitary Code 5th Edition, 362–364 (updated May 1988 as International Animal Health Code, updated pages 45 to 47).
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