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Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents (Text with EEA relevance)
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Article 4.Limitations based on the biodegradability of surfactants
Article 4a. Limitations on the content of phosphates and of other phosphorus compounds
PRIMARY BIODEGRADABILITY TEST METHODS FOR SURFACTANTS IN DETERGENTS
Primary biodegradability is measured by the determination in biodegraded liquors...
The pass criterion for primary biodegradability shall be a level...
The reference method for the laboratory testing of surfactants in...
ULTIMATE BIODEGRADABILITY (MINERALISATION) TEST METHODS FOR SURFACTANTS IN DETERGENTS
COMPLEMENTARY RISK ASSESSMENT FOR SURFACTANTS IN DETERGENTS
For those surfactants for which an environmental risk assessment is...
The complementary risk assessment run in the scope of this...
The study shall cover the aquatic environmental compartment. Additional information...
However, to minimise testing, and especially to avoid unnecessary animal...
1. Identity of the surfactant (in accordance with the provisions laid...
LIST OF SURFACTANTS THAT HAVE OBTAINED A DEROGATION
By way of derogation granted in accordance with Articles 4-6...
Name in the IUPAC nomenclature EC Number CAS number Limitations...
‘Einecs’ means the European Inventory of Existing Commercial Chemical Substances....
‘ELINCS’ means the European List of Notified Chemical Substances. The...
‘NLP’ means No-Longer Polymer. The term polymer is defined in...
TEST METHODS AND ANALYTICAL METHODS
The following test and analytical methods apply to control procedures...
2. Determination of anionic surfactants in biodegradability tests
3. Determination of non-ionic surfactants in biodegradation test liquors
3.2.3. Dilute hydrochloric acid [20 ml concentrated acid (HCl) diluted to...
3.2.4. Methanol AR, freshly distilled, stored in a glass bottle.
3.2.6. Precipitating agent: the precipitating agent is a mixture of two...
3.2.7. Glacial acetic acid 99-100 % (lower concentrations are unsuitable).
3.2.8. Ammonium tartrate solution: mix 12,4 g tartaric acid AR and...
3.2.9. Dilute ammonia solution: 40 ml ammonia solution AR (d =...
3.2.10. Standard acetate buffer: dissolve 40 g solid sodium hydroxide AR,...
3.2.11. Pyrrolidinedithiocarbamate solution (known as ‘carbate solution’): dissolve 103 mg sodium...
3.2.12. Copper sulphate solution (for standardisation of 3.2.11).
3.2.17. Gooch crucible, diameter of the perforated base = 25 mm,...
3.2.18. Circular glass-fibre filter papers, 27 mm diameter with fibre diameter...
3.2.19. Two filter flasks with adapters and rubber collars, 500 and...
3.2.20. Recording potentiometer fitted with a bright platinum indicator electrode and...
4. Preliminary treatment of anionic surfactants to be tested
4.3.2. Ethanol, 95 % (v/v) C2H5OH (permissible denaturant: methyl ethyl ketone...
4.3.4. Solution of carbon dioxide in ethanol (approximately 0,1 % CO2):...
4.3.5. Ammonium bicarbonate solution (60/40 v/v): 0,3 mol NH4HCO3 in 1...
4.3.6. Cation exchanger (KAT), strongly acidic, resistant to alcohol (50-100 mesh)...
4.3.7. Anion exchanger (AAT), macro-porous, Merck Lewatit MP 7080 (70-150 mesh)...
4.3.9. 2 000 ml round-bottomed flask with ground glass stopper and...
4.3.10. 90 mm diameter suction filter (heatable) for filter papers
4.3.12. Exchange columns with heating jacket and tap: inner tube 60...
4.4. Preparation of extract and separation of anionic active agents
5.2. From a homogeneous sample (powders, dried paste and dried liquids)...
5.3.1. Ethanol, C2H5OH 95 % (v/v) (permissible denaturant: methyl-ethyl ketone or...
5.3.4. 0,3 mol NH4HCO3 in 1 000 ml of an isopropanol/water...
5.3.5. Anion exchanger (AAT), macro-porous, Merck Lewatit MP 7080 (70-150 mesh)...
5.3.7. 2 000 ml round-bottomed flask with ground glass stopper and...
5.3.8. 90 mm diameter suction Filter (heatable) for filter papers
5.3.10. Exchange columns with heating jacket and tap: inner tube 60...
5.3.15. Preparation of extract and separation of non-ionic active agents
A Storage vessel B Dosing device C Aeration chamber (three...
A Running-in period B Period used for calculation (twenty-one days)...
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