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Commission Directive 2006/56/ECShow full title
Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot
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This is the original version (as it was originally adopted).
(a)General growth media
Nutrient agar (NA)
| Nutrient Agar (Difco) | 23,0 g |
| Distilled water | 1,0 L |
Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 min.
Nutrient dextrose agar (NDA)
Difco bacto nutrient agar containing 1 % D(+) glucose (monohydrate). Sterilize by autoclaving at 115 °C for 20 minutes.
Yeast peptone glucose agar (YPGA)
| Yeast Extract (Difco) | 5,0 g |
| Bacto-Peptone (Difco) | 5,0 g |
| D(+) Glucose (monohydrate) | 10,0 g |
| Bacto-Agar (Difco) | 15,0 g |
| Distilled water | 1,0 L |
Dissolve ingredients and sterilise by autoclaving at 121 °C for 15 min.
Yeast extract mineral salts medium (YGM)
| Bacto-Yeast-Extract (Difco) | 2,0 g |
| D(+) Glucose (monohydrate) | 2,5 g |
| K2HPO4 | 0,25 g |
| KH2PO4 | 0,25 g |
| MgSO4.7H2O | 0,1 g |
| MnSO4.H2O | 0,015 g |
| NaCl | 0,05 g |
| FeSO4.7H2O | 0,005 g |
| Bacto-Agar (Difco) | 18 g |
| Distilled water | 1,0 L |
Dissolve ingredients and sterilize 0,5 litre volumes of medium by autoclaving at 115 °C for 20 minutes.
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