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Commission Directive 2006/56/ECShow full title
Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot
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1.Buffers for extraction procedure
1.1.Extraction buffer (50 mM phosphate buffer, pH 7,0)
This buffer is used for extraction of the bacterium from plant tissues by homogenisation or shaking.
| Na2HPO4 (anhydrous) | 4,26 g |
| KH2PO4 | 2,72 g |
| Distilled water | 1.00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
Additional components may be useful as follows:
1.2.Pellet buffer (10 mM phosphate buffer, pH 7,2)
This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.
| Na2HPO4.12H2O | 2,7 g |
| NaH2PO4.2H2O | 0,4 g |
| Distilled water | 1,00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
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