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- Original (As adopted by EU)
Council Directive 93/85/EEC of 4 October 1993 on the control of potato ring rot
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This is the original version (as it was originally adopted).
Article 1.The Directive concerns the measures to be taken within the...
Article 2.(1) Member States shall conduct systematic official surveys for the...
Article 3.Member States shall ensure that the suspected occurrence or confirmed...
Article 4.(1) In cases of suspected occurrence, the responsible official bodies...
Article 5.(1) If official or officially supervised laboratory testing using the...
Article 6.Member States shall prescribe that where tubers or plants have...
Article 7.(1) Member States shall prescribe that tubers or plants, designated...
Article 8.(1) Member States shall prescribe that seed potatoes shall meet...
Article 9.Member States shall ban the holding and handling of the...
Article 10.Without prejudice to the provisions of Directive 77/93/EEC, Member States...
Article 11.Member States may adopt such additional or stricter measures as...
Article 12.Amendments to the Annexes to this Directive, to be made...
Article 13.(1) By 15 November 1993 Member States shall adopt and...
Article 14.Directive 80/665/EEC is hereby repealed with effect from 16 November...
Article 15.This Directive is addressed to the Member States.
3. Preparation of samples for Gram staining, immunofluorescence staining (IF) and...
5.2.1. Prepare three serial ten fold dilutions (101, 102, 103) of...
5.2.2. Pipette a measured standard volume sufficient to cover the window...
5.2.4. Cover appropriate windows which C. sepedonicum antiserum at the recommended...
5.2.5. Incubate in a humid chamber at ambient temperature for 30...
5.2.8. Cover each window with FITC conjugate at the same dilution...
5.2.10. Apply approximately 5 to 10 μl of 0,1 M phosphate...
5.2.11. Examine with a microscope fitted with an epifluorescent light source...
6.1. Distribute the pellet from 3.3 between at least 25 eggplants...
6.5. Inoculate 25 plants with a known C. sepedonicum culture and,...
6.7. Incubate the plants in appropriate conditions (Appendix 5) for 40...
6.8. Prepare a Gram stain (4) for all batches of eggplants...
6.9. Under certain circumstances, in particular where growing conditions are not...
FORMULATION OF MACERATING FLUID RECOMMENDED BY LELLIOTT AND SELLAR, 1976
GRAM STAIN PROCEDURE (HUCKER'S MODIFICATION) (DOETSCH, 1981)
DETERMINATION OF POPULATION OF IF-POSITIVE CELLS
Where D = diameter of window. Surface area(s) of objective...
Calculate d either by direct measurement or from the following...
Count the number of typical fluorescent cells per field (c)....
Calculate the number of typical fluorescent cells per window (C)....
Calculate the number of typical fluorescent cells per ml pellet...
NUTRITIONAL AND PHYSIOLOGICAL TESTS FOR THE IDENTIFICATION OF C. SEPEDONICUM
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