Search Legislation

Advanced Search

2003/422/EC: Commission DecisionShow full title

2003/422/EC: Commission Decision of 26 May 2003 approving an African swine fever diagnostic manual (Text with EEA relevance) (notified under document number C(2003) 1696)

Help about what version

What Version

Close

This is a legislation item that originated from the EU

After exit day there will be three versions of this legislation to consult for different purposes. The legislation.gov.uk version is the version that applies in the UK. The EU Version currently on EUR-lex is the version that currently applies in the EU i.e you may need this if you operate a business in the EU.

The web archive version is the official version of this legislation item as it stood on exit day before being published to legislation.gov.uk and any subsequent UK changes and effects applied. The web archive also captured associated case law and other language formats from EUR-Lex.

Changes to legislation:

There are outstanding changes not yet made to 2003/422/EC: Commission Decision. Those changes will be listed when you open the content using the Table of Contents below. Any changes that have already been made to the legislation appear in the content and are referenced with annotations. Help about Changes to Legislation

Close

Changes to Legislation

Revised legislation carried on this site may not be fully up to date. Changes and effects are recorded by our editorial team in lists which can be found in the ‘Changes to Legislation’ area. Where those effects have yet to be applied to the text of the legislation by the editorial team they are also listed alongside the affected provisions when you open the content using the Table of Contents below.

View outstanding changes

Changes and effects yet to be applied to :

Changes and effects yet to be applied to the whole legislation item and associated provisions

  1. Introductory Text

  2. Article 1.(1) The African swine fever diagnostic manual in the Annex...

  3. Article 2.This Decision shall apply from 1 July 2003.

  4. Article 3.This Decision is addressed to the Member States.

  5. Signature

    1. ANNEX

      AFRICAN SWINE FEVER DIAGNOSTIC MANUAL

      1. Chapter I Introduction, objectives and definitions

        1. 1. In order to ensure uniform procedures to diagnose African swine...

        2. 2. This manual is principally directed towards the authorities responsible for...

        3. 3. For the purpose of this manual, in addition to the...

      2. Chapter II Description of ASF with emphasis on differential diagnosis

        1. A. INTRODUCTION

          1. 1. ASF is caused by an enveloped DNA virus which belongs...

          2. 2. The ASF virus is very stable in excretions of infected...

          3. 3. The main natural route of infection of pigs in Europe...

          4. 4. The incubation period in individual animals is about five to...

          5. 5. Acute, subacute and chronic forms of ASF occur, the difference...

          6. 6. In pigs which clinically recover after infection, viraemia persists for...

        2. B. ACUTE FORM

          1. 1. The development of high fever (more than 40 °C) is...

          2. 2. In general the acute form of classical swine fever leads...

        3. C. SUBACUTE FORMS

        4. D. CHRONIC FORMS

      3. Chapter III Guidelines on main criteria to be considered for the recognition of a holding as an ASF suspected holding

        1. 1. The decision to recognise a holding as a suspected holding...

        2. 2. In any case, a holding must be considered as a...

      4. Chapter IV Checking and sampling procedures

        1. A. GUIDELINES AND PROCEDURES FOR CLINICAL EXAMINATION OF AND SAMPLING ON...

          1. 1. Member States shall ensure that appropriate clinical examinations, sampling and...

          2. 2. When an official veterinarian visits a suspected holding to confirm...

          3. 3. When reference is made to this paragraph, the clinical examination...

          4. 4. If dead or moribund pigs are detected in a suspected...

          5. 5. If further clinical signs or lesions that may suggest ASF...

          6. 6. If, after the examination carried out in a suspected holding,...

        2. B. SAMPLING PROCEDURES IN A HOLDING WHEN PIGS ARE KILLED FOLLOWING...

          1. 1. In order that the manner of introduction of the ASF...

          2. 2. The minimum number of pigs to be sampled must allow...

          3. 3. However, in case of secondary outbreaks, the competent authority may...

        3. C. SAMPLING PROCEDURES WHEN PIGS ARE KILLED AS A PREVENTIVE MEASURE...

          1. 1. In order that ASF may be confirmed or ruled out...

          2. 2. Sampling must primarily concern:

        4. D. CHECKING AND SAMPLING PROCEDURES BEFORE AUTHORISATION IS GIVEN TO MOVE...

          1. 1. Without prejudice to the provisions of Article 11(1)(f), second subparagraph,...

          2. 2. In the case of pigs to be moved to another...

          3. 3. In case of pigs to be moved to a slaughterhouse,...

          4. 4. When the pigs referred to in point 3 are slaughtered...

          5. 5. However, if clinical signs or post-mortem lesions suggesting ASF are...

          6. 6. The derogation provided for in Article 10(5) and Article 11(4)...

        5. E. CHECKING AND SAMPLING PROCEDURES IN A HOLDING IN RELATION TO...

          1. 1. When pigs are reintroduced into a holding in accordance with...

          2. 2. When pigs are reintroduced into a holding in accordance with...

          3. 3. After any reintroduction of pigs, the competent authority shall ensure...

        6. F. SAMPLING PROCEDURES IN HOLDINGS IN THE PROTECTION ZONE BEFORE LIFTING...

          1. 1. In order that the measures referred to in Article 10...

          2. 2. The minimum number of blood samples to be taken must...

        7. G. SAMPLING PROCEDURES IN HOLDINGS IN THE SURVEILLANCE ZONE BEFORE LIFTING...

          1. 1. In order that the measures referred to in Article 11...

          2. 2. Whenever blood sampling for serological tests is carried out in...

        8. H. SEROLOGICAL MONITORING AND SAMPLING PROCEDURES IN AREAS WHERE ASF IS...

          1. 1. In case of serological monitoring in feral pigs in areas...

          2. 2. If data on population density and size are not available,...

          3. 3. Without prejudice to the provisions of Article 15(2)(c) of Directive...

          4. 4. Collection of samples for virological tests from feral pigs shot...

          5. 5. All samples to be sent to the laboratory must be...

      5. Chapter V General procedures and criteria for collection and transport of samples

        1. A. GENERAL PROCEDURES AND CRITERIA

          1. 1. Before sampling is carried out in a suspected holding, a...

          2. 2. Each time that it is deemed that re-sampling of pigs...

          3. 3. All samples must be sent to the laboratory accompanied by...

        2. B. COLLECTION OF SAMPLES FOR VIROLOGICAL TESTS

          1. 1. For detection of the ASF virus, antigen or genome from...

          2. 2. Anticoagulated blood and/or clotted blood samples must be collected from...

        3. C. TRANSPORT OF SAMPLES

          1. 1. It is recommended that all samples:

          2. 2. The outside of the package must be labelled with the...

          3. 3. The responsible person in the laboratory receiving the samples must...

          4. 4. For air transport of samples to the Community Reference Laboratory...

      6. Chapter VI Principles and use of virological tests and evaluation of their results

        1. A. DETECTION OF VIRUS ANTIGENS

          1. 1. Direct immuno fluorescent test (DIFT)

          2. 2. ELISA for antigen detection

        2. B. VIRUS ISOLATION AND IDENTIFICATION BY THE HAEMADSORPTION TEST (HAD)

          1. 1. Virus isolation is based on the inoculation of sample material...

          2. 2. The HAD technique is recommended for the identification of ASF...

          3. 3. Virus isolation is best suited for the investigation of samples...

          4. 4. Virus isolation and identification by HAD are recommended as a...

          5. 5. All ASF virus isolates from all primary outbreaks, primary cases...

        3. C. DETECTION OF THE VIRUS GENOME

          1. 1. The polymerase chain reaction (PCR) is applied to detect the...

          2. 2. PCR can be used on a limited number of samples...

          3. 3. Suitable sample material for the PCR are the organs described...

          4. 4. The PCR can be performed within a working day. It...

        4. D. RECOMMENDED VIROLOGICAL TESTS AND EVALUATION OF THE RESULTS

        5. E. GENETIC CHARACTERISATION OF ASF VIRUS ISOLATES

          1. 1. Genetic characterisation of ASF virus isolates is achieved by determining...

          2. 2. If virus molecular characterisation cannot be performed in a national...

      7. Chapter VII Principles and use of serological tests and evaluation of their results

        1. A. BASIC PRINCIPLES AND DIAGNOSTIC VALUE

          1. 1. ASF-specific antibody detection is recommended for subacute and chronic forms...

          2. 2. The detection of antibodies against the ASF virus in serum...

        2. B. RECOMMENDED SEROLOGICAL TESTS

          1. 1. The ELISA, indirect immunofluorescence test (IIFT) and immunoblotting (IB) tests...

          2. 2. The ELISA test is the most reliable and useful test...

          3. 3. Quality control on sensitivity and specificity of each batch of...

          4. 4. The IIFT is a rapid technique with high sensitivity and...

          5. 5. The IB test is a highly specific and sensitive technique...

      8. Chapter VIII Minimum safety requirements for ASF laboratories

        1. 1. The requirements laid down in table 1 must be fulfilled...

        2. 2. The requirements laid down in table 2 must be fulfilled...

        3. 3. All stocks of the ASF virus must be kept in...

        4. 4. It is recommended that the biosecurity unit for ASF virus...

Back to top

Options/Help

Print Options

Close

Legislation is available in different versions:

Latest Available (revised):The latest available updated version of the legislation incorporating changes made by subsequent legislation and applied by our editorial team. Changes we have not yet applied to the text, can be found in the ‘Changes to Legislation’ area.

Original (As adopted by EU): The original version of the legislation as it stood when it was first adopted in the EU. No changes have been applied to the text.

Close

Opening Options

Different options to open legislation in order to view more content on screen at once

Close

More Resources

Access essential accompanying documents and information for this legislation item from this tab. Dependent on the legislation item being viewed this may include:

  • the original print PDF of the as adopted version that was used for the EU Official Journal
  • lists of changes made by and/or affecting this legislation item
  • all formats of all associated documents
  • correction slips
  • links to related legislation and further information resources
Close

Timeline of Changes

This timeline shows the different versions taken from EUR-Lex before exit day and during the implementation period as well as any subsequent versions created after the implementation period as a result of changes made by UK legislation.

The dates for the EU versions are taken from the document dates on EUR-Lex and may not always coincide with when the changes came into force for the document.

For any versions created after the implementation period as a result of changes made by UK legislation the date will coincide with the earliest date on which the change (e.g an insertion, a repeal or a substitution) that was applied came into force. For further information see our guide to revised legislation on Understanding Legislation.

Close

More Resources

Use this menu to access essential accompanying documents and information for this legislation item. Dependent on the legislation item being viewed this may include:

  • the original print PDF of the as adopted version that was used for the print copy
  • correction slips

Click 'View More' or select 'More Resources' tab for additional information including:

  • lists of changes made by and/or affecting this legislation item
  • confers power and blanket amendment details
  • all formats of all associated documents
  • links to related legislation and further information resources