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10. After the last wash each embryo shall be subjected to microscopic examination at a magnification of at least 50x over its entire surface to determine that the zona pellucida is intact and is free from any adherent material. Any micro-manipulation which involves penetration of the zona pellucida must be carried out after the last wash and examination in the facilities approved for the purpose. Such micro-manipulation may only be carried out on an embryo having an intact zona pellucida.
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