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Weigh accurately into a 100 ml standard flask a quantity of the product sufficient to contain 10,0 mg of anhydrous quinine, add 20 ml of methanol (4.6) and place the flask in an ultrasonic bath (5.2) for 20 minutes. Make up to the mark with methanol (4.6). Mix the solution and then filter an aliquot (5.5).
Flowrate: 1,0 ml/min.
Detector wavelength (5.3): 332 nm.
Injection volume: 10 μl of filtered solution (6.1).
Measurement: peak area.
Inject at least three times 10,0 μl of each reference solution (4.12), measure the area of the peaks, and calculate the average area at each concentration.
Produce the calibration curve and verify that it is rectilinear.
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