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The Private Water Supplies (England) Regulations 2016
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PART 2Analytical methods
Table 1
Prescribed methods of analysis
| Parameter | Method |
|---|---|
| Clostridium perfringens (including spores) | Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds. |
| Coliform bacteria and Escherichia coli (E. coli) | BS-EN ISO 9308-1 and BS-EN ISO 9308-2 |
Colony count 22°C-enumeration of culturable microorganisms | BS-EN ISO 6222 |
Colony count 37°C-enumeration of culturable microorganisms | BS-EN ISO 6222 |
| Enterococci | BS-EN ISO 7899-2 |
| Pseudomonas aeruginosa | BS-EN ISO 12780 |
| *Use the following method to make m-CP agar: | ||
| Make a basal medium consisting of— | ||
| Tryptose | 30.0g | |
| Yeast extract | 20.0g | |
| Sucrose | 5.0g | |
| L-cysteine hydrochloride | 1.0g | |
| MgSO4.7H2O | 0.1g | |
| Bromocresol purple | 40.0mg | |
| Agar | 15.0g | |
| Water | 1,000.0ml | |
| Dissolve the ingredients of the basal medium; adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool. | ||
| Dissolve— | ||
| D-cycloserine | 400.0mg | |
| Polymyxine-B sulphate | 25.0mg | |
| Indoxyl-β-D-glucoside | 60.0mg | |
| into 8ml sterile water and add it to the medium. | ||
| Add to the medium— | ||
| Filter-sterilised 0.5% phenolphthalein diphosphate solution | 20.0ml | |
| Filter-sterilised 4.5% FeCl3.6H2O | 2.0ml | |
Table 2
Prescribed performance characteristics for methods of analysis
| Parameters | Trueness % of prescribed concentration or value or specification | Precision % of prescribed concentration or value or specification | Limit of detection % of prescribed concentration or value or specification |
|---|---|---|---|
(i) The method of analysis should determine total cyanide in all forms | |||
(ii) The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned. | |||
(iii) The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Part 1 of Schedule 1. | |||
(iv) The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Part 1 of Schedule 1. | |||
(v) The performance characteristics apply to the prescribed value of 4 NTU. | |||
(vi) The performance characteristic apply to the specification of 1 NTU for surface waters or ground waters influenced by surface water. | |||
| Aluminium | 10 | 10 | 10 |
| Ammonium | 10 | 10 | 10 |
| Antimony | 25 | 25 | 25 |
| Arsenic | 10 | 10 | 10 |
| Benzene | 25 | 25 | 25 |
| Benzo(a)pyrene | 25 | 25 | 25 |
| Boron | 10 | 10 | 10 |
| Bromate | 25 | 25 | 25 |
| Cadmium | 10 | 10 | 10 |
| Chloride | 10 | 10 | 10 |
| Chromium | 10 | 10 | 10 |
| Colour | 10 | 10 | 10 |
| Conductivity | 10 | 10 | 10 |
| Copper | 10 | 10 | 10 |
| Cyanide(i) | 10 | 10 | 10 |
| 1,2-dichloroethane | 25 | 25 | 10 |
| Fluoride | 10 | 10 | 10 |
| Iron | 10 | 10 | 10 |
| Lead | 10 | 10 | 10 |
| Manganese | 10 | 10 | 10 |
| Mercury | 20 | 10 | 20 |
| Nickel | 10 | 10 | 10 |
| Nitrate | 10 | 10 | 10 |
| Nitrite | 10 | 10 | 10 |
| Pesticides and related products(ii) | 25 | 25 | 25 |
| Polycyclic aromatic hydrocarbons(iii) | 25 | 25 | 25 |
| Selenium | 10 | 10 | 10 |
| Sodium | 10 | 10 | 10 |
| Sulphate | 10 | 10 | 10 |
| Tetrachloroethene(iv) | 25 | 25 | 10 |
| Tetrachloromethane | 20 | 20 | 20 |
| Trichloroethene(iv) | 25 | 25 | 10 |
| Trihalomethanes: Total(iii) | 25 | 25 | 10 |
| Turbidity(v) | 10 | 10 | 10 |
| Turbidity(vi) | 25 | 25 | 25 |
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