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4.—4.1 250 ml conical flasks with ground glass or PTFE stoppers.
4.2 Stoppered centrifuge tubes.
4.3 Gas chromatograph with flame ionisation detector.
4.4 Column:
either (i) 1.5 m glass column (4 mm internal diameter) packed with 5% QV17 on Gas Chrom Q, 80–100 mesh,
or (ii) 1.5 m glass column (4 mm internal diameter) packed with 5% Carbowax 20M-TPA on Diatomite C-AAW, 80–100 mesh.
4.5 Water bath: hotplate stirrer on which is placed a 2,000 ml beaker (or suitable vessel) containing water maintained at 40–50°C.
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