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Commission Regulation (EC) No 152/2009Show full title
Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)
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8.ObservationsU.K.
8.1.For products with a high content of oils and fats, which are difficult to crush or unsuitable for drawing a homogeneous reduced test sample, proceed as follows.U.K.
Weigh 20 g of the sample to the nearest 1 mg and mix with 10 g or more of anhydrous sodium sulfate (3.2). Extract with light petroleum (3.1) as indicated in point 5.1. Make up the extract obtained to 500 ml with light petroleum (3.1) and mix. Take 50 ml of the solution and place in a small, dry, weighed flask containing fragments of pumice stone. Distil off the solvent, dry and proceed as indicated in the last paragraph of point 5.1.
Eliminate the solvent from the extraction residue left in the thimble, crush the residue to a fineness of 1 mm, return it to the extraction thimble (do not add sodium sulfate) and proceed as indicated in the second and third paragraphs of point 5.1.
Calculate the content of oils and fats as a percentage of the sample by using the following formula:
(10m1 + m2) × 5
where:
m1
=
weight in grams of the residue after the first extraction (aliquot part of the extract),
m2
=
weight in grams of the residue after the second extraction.
8.2.For products low in oils and fats the test sample may be increased to 5 g.U.K.
8.3.Pet foods containing a high content of water may need to be mixed with anhydrous sodium sulfate prior to hydrolysis and extraction as per Procedure B.U.K.
8.4.In paragraph 5.2 it may be more effective to use hot water in place of cold water to wash the residue after filtration.U.K.
8.5.The drying time of 1,5 h may need to be extended for some feed. Excessive drying shall be avoided as this can lead to low results. A microwave oven can also be used.U.K.
8.6.Pre-extraction by Procedure A prior to hydrolysis and re-extraction by Procedure B is recommended if the crude oil/fat content is greater than 15 %. To some extent this depends on the nature of the feed and the nature of the oil/fat in the feed.U.K.
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