SCHEDULE 2Authorisation of a preparation of Lactobacillus buchneri DSM 29026 (identification number 1k20759) as a feed additive for all animal species

Regulation 3(1)

The preparation specified in the table, belonging to the additive category “technological additives” and to the functional group “silage additives”, is authorised as an additive in animal nutrition subject to the conditions set out in the table.

Additive

Lactobacillus buchneri DSM 29026

Identification number of the additive

1k20759

Authorisation holder

None specified

Additive category

Technological additives

Functional group

Silage additives

Additive composition

Preparation of Lactobacillus buchneri DSM 29026 containing a minimum of 2 × 1010 CFU/g additive

Characterisation of the active substance(s)

Viable cells of Lactobacillus buchneri DSM 29026

Analytical methods(1)

For enumeration (colony count) of the feed additive:

  • Spread plate method on MRS agar (BS EN 15787:2021(2)).

For identification of bacterial strain:

  • Pulsed Field Gel Electrophoresis (PFGE).

Species or category of animal

All animal species

Maximum age

None

Minimum content(3)

Minimum content of the feed additive when not combined with other micro-organisms as silage additives: 5 × 107 CFU/kg of easy and moderately difficult to ensile fresh material(4)

Maximum content(3)

None

Other provisions

In the directions for use of the feed additive and premixtures, the storage conditions must be indicated.

(1)

Details of the analytical methods set out in the document referenced “Ares(2019)4747322 - 22/07/2019” and last updated 18 October 2019. The document is available at the following address: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports/fad-2018-0093.

(2)

Under reference BS EN 15787:2021 “Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Lactobacillus spp. used as feed additive”. Published by the British Standards Institution on 31 December 2021 (ISBN 978 0 580 99831 7). Available from the British Standards Institution https://knowledge.bsigroup.com.

(3)

Content of element Lactobacillus buchneri DSM 29026 in CFU of additive/kg of fresh material.

(4)

Easy to ensile forage: > 3% soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1.5-3.0% soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No 429/2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives13.