SCHEDULE 3E+W+SSAMPLING AND TESTING METHODS

PART IIIE+W+SMETHODS FOR THE ISOLATION OF SALMONELLA

A. BACTERIOLOGICAL METHODE+W+S

Day 5E+W+S

8.  The incubated composite media or equivalent shall be examined and the findings recorded, discarding cultures which are obviously not Salmonella. Slide serological tests shall be performed using Salmonella polyvalent “O” and polyvalent “H” (phase 1 and 2) agglutinating sera on selected suspect colonies collected from the blood agar or MacConkey plates. If reactions occur with one or both sera, the colonies shall be typed by slide serology and a subculture sent (in Scotland) to the Veterinary Laboratory, Lasswade, Midlothian and (in England and Wales) to a Veterinary Investigation Centre of the Ministry of Agriculture, Fisheries and Food for further typing.