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7.3 According to the presumed biuret content, transfer with a pipette 25 or 50 ml from the solution prepared in 7.2, to a 100 ml graduated flask and neutralise if necessary with 0.05 M sulfuric acid or sodium hydroxide solution (4.2 or 4.3) as required, using methyl red indicator (4.7). Add 20.0 ml of the alkaline solution of potassium sodium tartrate (4.4) and 20.0 ml of the copper solution (4.5). Make up to volume, mix thoroughly and leave standing for 15 minutes at 30 C+2. Measure the absorbance of the solution as described in 7.1.
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