7.6—7.6.1 Transfer by pipette into a 500 ml graduated flask, a portion of filtrate (7.1) containing not more than 250 mg ureic nitrogen. To remove phosphates, add a suitable quantity of saturated barium hydroxide solution (4.18) until further addition does not cause the production of more precipitate. Eliminate excess barium ions (and any dissolved calcium ions) by means of 10% sodium carbonate solution (4.19). Allow to settle and check whether precipitation is complete. Make up to the mark, mix and filter through a fluted filter. Transfer by pipette 50 ml filtrate into the 300 ml Erlenmeyer flask of the apparatus (5.3). Acidify with 2 N hydrochloric acid (4.20) to pH 3.0, measured by means of the pH meter (5.5). Raise the pH to 5.4 by the addition of 0.1 N sodium hydroxide (4.17). To avoid ammonia losses when hydrolysis by urease occurs, close the Erlenmeyer flask by means of a stopper provided with a dropping funnel and a small bubble trap containing exactly 2 ml standard 0.1 N hydrochloric acid solution (4.21). Introduce through the separating funnel, 20 ml urease solution (4.22). Allow to stand for one hour at 20 — 25°C. Place 25 .O ml of the standard 0.1 N hydrochloric acid solution (4.2) in the dropping funnel, allow to run into the L solution, then rinse with a little water. Transfer quantitatively the contents of the bubble trap to the solution contained in the Erlenmeyer. Titrate the excess acid using the standard 0.1 N sodium hydroxide solution (4.17), until a pH of 5.4 is obtained, measured on the pH meter.