    1 U is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from a sodium phytate substrate at pH 5,5 and 37 °C.    ,
    Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports    ,



4a24,Danisco (UK) Ltd,6-phytase EC 3.1.3.26,   Additive composition    Preparation of 6-phytase produced by Trichoderma reesei (ATCC SD-6528) having a minimum activity of 15 000 U /g.    Liquid form      Characterisation of the active substance    6-phytase (EC 3.1.3.26) produced by Trichoderma reesei (ATCC SD-6528)      Analytical method     For the quantification of 6-phytase activity in the feed additive:       colorimetric method based on the enzymatic reaction of phytase on the phytate.       For the quantification of 6-phytase activity in premixtures and feedingstuffs:       colorimetric method based on the enzymatic reaction of phytase on the phytate EN ISO 30024.      ,  All poultry species    All porcine species (other than suckling piglets)  ,—,250 U,—,  1.   In the directions for use of the additive and premixture the storage conditions and stability to pelleting shall be indicated.     2.   Maximum recommended dose: 2 000 U/kg feed.     3.   For users of the additive and premixtures in a feed business, operational procedures and appropriate organisational measures shall be established to address hazards by inhalation, dermal contact or eye contact. Where the dermal, inhalator or eye exposure cannot be reduced to an acceptable level by these procedures and measures, the additive and premixtures shall be used with appropriate personal protective equipment.   ,29 June 2026,
