    Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx    ,



4d 3,Vetagro SpA,Preparation of citric acid, sorbic acid, thymol and vanillin,     Additive composition     Preparation of protected microbeads containing citric acid, sorbic acid, thymol and vanillin with a minimum of:      Citric acid: 25 g/100 g      Sorbic acid: 16,7 g/100 g      Thymol: 1,7 g/100 g      Vanillin: 1 g/100 g         Characterisation of active substances       Citric acid C 6 H 8 O 7 (purity ≥ 99,5 %)    2-hydroxy-1,2,3-propanetricarboxylic acid, CAS number 77-92-9 anhydrous      Sorbic acid C 6 H 8 O 2 (purity ≥ 99,5 %)    2,4-hexadienoic acid, CAS number 110-44-1      Thymol (purity ≥ 98 %)    5-methyl-2-(1-methylethyl)phenol, CAS number 89-83-8)      Vanillin (purity ≥ 99,5 %)    4-hydroxy-3-methoxybenzaldehyde, CAS number 121-33-5)         Analytical methods      Determination of sorbic acid and thymol in feed: reverse phase high performance liquid chromatography method equippedwith ultraviolet/diode array detection (RP-HPLC-UV/DAD). Determination of citric acid in the additive and premixtures: (RP-HPLC-UV/DAD). Determination of citric acid in feedingstuff: enzymatic determination of citric content-NADH (reduced form of nicotinamide adenine dinucleotide) spectrometric method.    ,  Chickens for fattening and reared for laying    Minor avian species for fattening and reared for laying  ,—,200,—,For safety: breathing protection, glasses and gloves shall be used during handling.,10 October 2022,
  Weaned Suidae     other than Sus scrofa domesticus   ,1 000,
