1 U is the amount of the enzyme which liberates 1 μmol of p-nitrolphenol per minute from p-nitrophenyl-alpha-galactopyranoside (pNPG) at pH 5,0 and 37 °C.    ,
    1 U is the amount of the enzyme which liberates 1 mg of reducing sugar (glucose equivalent) per minute from beta-glucan at pH 5,0 and 50 °C.    ,
    Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx    ,



4a17,Kerry Ingredients and Flavours,  Alpha-galactosidase    (EC 3.2.1.22)    Endo-1,4-beta-glucanase    (EC 3.2.1.4)  ,     Additive composition     Preparation of alpha-galactosidase (EC 3.2.1.22) produced by Saccharomyces cerevisiae (CBS 615.94) and endo-1,4-beta-glucanase (EC 3.2.1.4) produced by Aspergillus niger (CBS 120604), solid form, with a minimum activity of:      1 000 U alpha-galactosidase/g      5 700 U endo-1,4-beta-glucanase/g         Characterisation of the active substance     Alpha-galactosidase produced by Saccharomyces cerevisiae (CBS 615.94) Endo-1,4-beta-glucanase produced by Aspergillus niger (CBS 120604)       Method of Analysis      Determination:      colorimetric method measuring p-nitrophenol released by action of alpha-galactosidase from p-nitrophenyl-alpha-galactopyranoside substrate,      colorimetric method measuring water soluble dye released by action of endo-1,4-beta-glucanase from azurine-crosslinked barley glucan substrate.      ,Chickens for fattening,—,  50 U alpha-galactosidase    285 U endo-1,4-beta-glucanase  ,—,  1.   In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.     2.  Maximum recommended dose:      100 U alpha-galactosidase/kg      570 U endo-1,4-beta-glucanase/kg.        3.   For safety: breathing protection, glasses and gloves shall be used during handling.   ,9 April 2022,