Details of the analytical methods are available at the following address of the European Union Reference Laboratory for Feed Additives: www.irmm.jrc.be/eurl-feed-additives    ,



5 1 770,Alpharma (Belgium) BVBA,  Maduramicin ammonium alpha 10 g/kg    (Cygro 10 G)  ,     Additive Composition     Maduramicin ammonium alpha: 10 g/kg    Carboxymethylcellulose sodium: 20 g/kg    Calcium sulphate dihydrate: 970 g/kg       Active substance     Maduramicin ammonium alpha C 47 H 83 O 17 N    CAS number: 84878-61-5    (2R, 3S, 4S, 5R, 6S)-6[(1R)-1- [(2S,5R,7S,8R,9S)-2-    [2S,2′R,3′S,5R,5    ′R)-3′-[(2,6-dideoxy-3,4-di-    O-methyl-β-L-arabinohexopyra    nosyl)oxy]-octahydro-2-    methyl-5′-[(2S,3S,5R,6S)-    tetrahydro-6-hydroxy-3,5,6-    trimethyl-2H-pyran-    2-yl][2,2′-bifuran]-5-yl]-9-    hydroxy-2,8-dimethy-    1,6dioxaspirol[4.5]dec-7-    yl]ethyl]tetrahydro-    2-hydroxy-4,5-    dimethoxy-3-    methyl-3H-pyran-2-acetic    acid, monoammonium    salt produced by a fermentation process by the strain Actinomadura      yumaensis NRRL 12515: ≥ 90 %.    Related impurities:    Maduramicin ammonium β: ≤ 1 %       Analytical method      For the determination of maduramicin ammonium alpha in the additive, premixtures and feedingstuffs: reversed-phased high performance liquid chromatography (HPLC) using post-column derivatization with vanillin and detection at 520nm- EN 15781:2009.    For the determination of residues of maduramicin ammonium alpha in liver and muscle: reversed-phased high performance liquid chromatography (HPLC) coupled to tandem mass spectrometry.    ,Chickens for fattening,—,5,6,  1.   The additive shall be incorporated in compound feed in form of a premixture.     2.   Maduramicin ammonium alpha shall not be mixed with other coccidiostats.     3.  Indicate in the instructions for use: ‘Dangerous for equines’.   ‘This feedingstuff contains an ionophore: simultaneous use with certain medicinal substances (e.g. tiamulin) can be contraindicated’.     4.   For safety: breathing protection, glasses and gloves shall be used during handling.     5.   A post-market monitoring program on the resistance to Eimeria spp. shall be planned and executed by the holder of authorisation.     6.   The use is prohibited at least 3 days before slaughter   ,10 May 2021,  150 μg of maduramycin ammonium/kg of fresh liver, skin and fat;    100 μg of maduramycin ammonium/kg of fresh kidney;    30 μg of maduramycin ammonium/kg of fresh muscle.  ,