Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance) (c. 152)

ANNEX IV U.K. METHODS OF ANALYSIS TO CONTROL THE LEVEL OF AUTHORISED ADDITIVES IN FEED

A. DETERMINATION OF VITAMIN A U.K.

4. Apparatus U.K.

4.1. Vacuum rotary evaporator U.K.

4.2. Amber glassware U.K.

4.2.1. Flat bottom or conical flasks, 500 ml, with ground-glass socket U.K.

4.2.2. Graduated flasks with ground-glass stoppers, narrow-necked, 10, 25, 100 and 500 ml U.K.

4.2.3. Separating funnels, conical, 1 000 ml, with ground-glass stoppers U.K.

4.2.4. Pear shaped flasks, 250 ml, with ground-glass sockets U.K.

4.3. Allihn condenser, jacket length 300 mm, with ground-glass joint, with adapter for gas feed pipe U.K.

4.4. Pleated filter paper for phase separation, diameter 185 mm (e.g. Schleicher & Schuell 597 HY 1/2) U.K.

4.5. HPLC equipment with injection system U.K.

4.5.1. Liquid chromatographic column, 250 mm x 4 mm, C₁₈, 5 or 10 μm packing, or equivalent (performance criterion: only a single peak for all retinol isomers under the HPLC-conditions) U.K.

4.5.2. UV or fluorescence detector, with variable wavelength adjustment U.K.

4.6. Spectrophotometer with 10 mm quartz cells U.K.

4.7. Water-bath with magnetic stirrer U.K.

4.8. Extraction apparatus (see figure 1) consisting of: U.K.

4.8.1. Glass cylinder of 1 l capacity fitted with a ground glass neck and stopper U.K.

4.8.2. Ground glass insert equipped with a side-arm and an adjustable tube passing through the centre. The adjustable tube shall have a U-shaped lower end and a jet at the opposite end so that the upper liquid layer in the cylinder may be transferred into a separating funnel. U.K.