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ANNEX VIIIU.K.TEST METHODS AND ANALYTICAL METHODS

3.Determination of non-ionic surfactants in biodegradation test liquorsU.K.

3.4.Calculation of resultsU.K.

Every non-ionic surfactant has its own factor, depending on its composition, particularly on the length of the alkene oxide chain. The concentration of non-ionic surfactant is expressed in relation to a standard substance — a nonyl phenol with ten ethylene oxide units (NP 10) — for which the conversion factor is 0,054.

Using this factor the amount of surfactant present in the sample is found expressed as mg of NP 10 equivalent, as follows:

(b — c) xfx 0,054 = mg non-ionic surfactant as NP 10

where:

b

=

volume of ‘carbate solution’ used by the sample (ml),

c

=

volume of ‘carbate solution’ used by the blank (ml),

f

=

factor of the ‘carbate solution’.