ANNEX I TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al.
SCOPE OF THE TEST SCHEME

7. BIOASSAY TEST
7.1. Distribute the whole of the remaining test aliquot of the resuspended pellet from section 3.1.6 or 3.2.5 between eggplants by one of the methods given below (7.3 or 7.4). Use only plants at leaf stage two to three up to full expansion of the third true leaf. In order to ensure complete utilisation of the resuspended pellet as well as effective inoculation the procedures outlined below will require 15 to 25 eggplants per sample.