NCP-88 mediumU.K.
| Nutrient agar (Difco) | 23 g |
| Yeast extract (Difco) | 2 g |
| D-mannitol | 5 g |
| K2HPO4 | 2 g |
| KH2PO4 | 0,5 g |
| MgSO4.7H2O | 0,25 g |
| Distilled water | 1,0 L |
Dissolve ingredients, adjust pH to 7,2. After autoclaving and cooling down to 50 °C, add the following antibiotics: Polymyxin B sulphate (Sigma) 0,003 g, nalidixic acid (Sigma) 0,008 g, Cycloheximide (Sigma) 0,2 g.
Dissolve antibiotics in stock solutions as follows: nalidixic acid in 0,01 M NaOH, cycloheximide in 50 % ethanol, polymyxin B sulphate in distilled water. Stock solutions are filter-sterilized.
Note:U.K.
Durability of basal medium is three months. After antibiotics are added durability is one month when stored refrigerated.