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[ANNEX II U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.
Appendix 6 Validated PCR protocols and reagents
5. Preparation of the loading buffer U.K.
5.1. Bromphenol blue (10 %- stock solution ) U.K.
Bromphenol blue | 5 g |
Distilled water (bidest) | 50 ml |
5.2. Loading buffer U.K.
Glycerol (86 %) | 3,5 ml |
Bromphenol blue (5,1) | 300 µl |
Distilled Water (bidest) | 6,2 ml] |