Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al. (c. 57)

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[F1 ANNEX II U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.

Textual Amendments

F1 Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..

Appendix 6 Validated PCR protocols and reagents

5. Preparation of the loading buffer U.K.

5.1. Bromphenol blue (10 %- stock solution ) U.K.

Bromphenol blue	5 g
Distilled water (bidest)	50 ml

5.2. Loading buffer U.K.

Glycerol (86 %)	3,5 ml
Bromphenol blue (5,1)	300 µl
Distilled Water (bidest)	6,2 ml]