[F13.1. Double strength coating buffer, pH 9,6. U.K.
Na 2 CO 3 | 6,36 g |
NaHCO 3 | 11,72 g |
Distilled water | 1,00 l |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 minutes.
Sodium sulphite (0.2 %) may be added as antioxidant if required to prevent build up of oxidised aromatic compounds.]