Council Directive 98/57/ECShow full title

Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al.

[F13.1. Double strength coating buffer, pH 9,6. U.K.
Na 2 CO 3 6,36 g
NaHCO 3 11,72 g
Distilled water 1,00 l

Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 minutes.

Sodium sulphite (0.2 %) may be added as antioxidant if required to prevent build up of oxidised aromatic compounds.]