Council Directive 98/57/ECShow full title

[F1 1. Buffers for extraction procedure U.K.

1.1. Extraction buffer (50 mM phosphate buffer, pH 7,0) U.K.

This buffer is used for extraction of the bacterium from plant tissues by homogenisation or shaking.

Na 2 HPO 4 (anhydrous)	4,26 g
KH 2 PO 4	2,72 g
Distilled water	1.00 l

Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.

Additional components may be useful as follows:

a For use with homogenisation extraction method
	Purpose	Quantity (per l)
Lubrol flakes	Deflocculant a	0,5 g
DC silicone antifoam	Anti-foam agent a	1,0 ml
Tetrasodium pyrophosphate	Anti-oxidant	1,0 g
Polyvinylpyrrolidone-40000 (PVP-40)	Binding of PCR inhibitors	50 g

1.2. Pellet buffer (10 mM phosphate buffer, pH 7,2) U.K.

This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.

Na 2 HPO 4 .12H 2 O	2,7 g
NaH 2 PO 4 .2H 2 O	0,4 g
Distilled water	1,0 l

Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 minutes.]

Textual Amendments

F1 Substituted by Commission Directive 2006/63/CE of 14 July 2006 amending Annexes II to VII to Council Directive 98/57/EC on the control of Ralstonia solanacearum (Smith) Yabuuchi et al..