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[F1 ANNEX I U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. SCOPE OF THE TEST SCHEME

Textual Amendments

F1 Substituted by Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot.

Appendix 6 Validated PCR protocol and reagents

3. 10x Tris Acetate EDTA (TAE) buffer, pH 8,0 U.K.

Tris buffer	48,4 g
Glacial acetic acid	11,42 ml
EDTA (disodium salt)	3,72 g
Distilled water	1,00 L

Dilute to 1x before use.

Also commercially available (e.g. Invitrogen or equivalent).]