Introductory Text
CHAPTER 1 General provisions
Article 1.. . . . . . . . . ....
Article 2.. . . . . . . . . ....
CHAPTER II Rules for the movement of equidae
Article 3.. . . . . . . . . ....
Article 4.. . . . . . . . . ....
Article 5.. . . . . . . . . ....
Article 6.. . . . . . . . . ....
Article 7.. . . . . . . . . ....
Article 8.. . . . . . . . . ....
Article 9.. . . . . . . . . ....
Article 10.. . . . . . . . . ....
CHAPTER III Rules for imports from third countries
Article 11.. . . . . . . . . ....
Article 12.. . . . . . . . . ....
Article 13.. . . . . . . . . ....
Article 14.. . . . . . . . . ....
Article 15.. . . . . . . . . ....
Article 16.. . . . . . . . . ....
Article 17.. . . . . . . . . ....
Article 18.. . . . . . . . . ....
Article 19.. . . . . . . . . ....
Article 20.. . . . . . . . . ....
Article 21.. . . . . . . . . ....
CHAPTER IV Final provisions
Article 22.. . . . . . . . . ....
Article 23.. . . . . . . . . ....
Article 24.. . . . . . . . . ....
Article 25.. . . . . . . . . ....
Article 26.. . . . . . . . . ....
Article 27.. . . . . . . . . ....
Article 28.. . . . . . . . . ....
ANNEX A
COMPULSORILY NOTIFIABLE DISEASES
. . . . . . . . . ....
ANNEX B
ANNEX C
ANNEX D
AFRICAN HORSE SICKNESS
1. COMPETITIVE ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...
1.1. Test procedure
1.1.1. Preparation of plates
1.1.1.1. . . . . . . . . . ....
1.1.1.2. . . . . . . . . . ....
1.1.2. Control wells
1.1.2.1. . . . . . . . . . ....
1.1.2.2. . . . . . . . . . ....
1.1.2.3. . . . . . . . . . ....
1.1.2.4. . . . . . . . . . ....
1.1.3. Spot test method
1.1.3.1. . . . . . . . . . ....
1.1.4. Serum titration method
1.1.4.1. . . . . . . . . . ....
1.1.5. Add 50 μl of guinea pig antisera, pre-diluted in blocking buffer,...
1.1.5.1. . . . . . . . . . ....
1.1.5.2. . . . . . . . . . ....
1.1.5.3. . . . . . . . . . ....
1.1.5.4. . . . . . . . . . ....
1.1.5.5. . . . . . . . . . ....
1.1.6. Chromogen
1.1.7. Reading
1.2. Expression of results
1.2.1. Using a software package print out the optical density (OD)...
1.2.2. The diagnostic threshold (cut-off value) for test sera is 50 %...
2. INDIRECT ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...
2.1. Test procedure
2.1.1. Solid phase
2.1.1.1. . . . . . . . . . ....
2.1.1.2. . . . . . . . . . ....
2.1.1.3. . . . . . . . . . ....
2.1.1.4. . . . . . . . . . ....
2.1.2. Test samples
2.1.2.1. Serum samples to be tested, and positive and negative control...
2.1.2.2. . . . . . . . . . ....
2.1.3. Conjugate
2.1.3.1. . . . . . . . . . ....
2.1.3.2. . . . . . . . . . ....
2.1.4. Cromogen/Substrate
2.1.4.1. Add 200 μl/well of chromogen/substrate solution (10 ml of 80,6 mM DMAB (dimethyl...
2.1.4.2. . . . . . . . . . ....
2.2. Interpretation of the results
2.2.1. . . . . . . . . . ....
2.2.2. . . . . . . . . . ....
2.2.3. . . . . . . . . . ....
2.2.4. . . . . . . . . . ....
3. BLOCKING ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...
3.1. Test procedure
3.1.1. ELISA plates
3.1.1.1. . . . . . . . . . ....
3.1.1.2. . . . . . . . . . ....
3.1.1.3. . . . . . . . . . ....
3.1.2. Test samples and controls
3.1.2.1. . . . . . . . . . ....
3.1.2.2. . . . . . . . . . ....
3.1.3. Conjugate
3.1.4. . . . . . . . . . ....
3.1.5. Chromogen/Substrate
3.1.6. Reading
3.2. Interpretation of the results
3.2.1. Assay validation
3.2.2. Cut-off calculation
3.2.3. Interpretation of results