Council Directive of 26 June 1990 on animal health conditions governing the movement and import from third countries of equidae (90/426/EEC) (repealed)

  1. Introductory Text

  2. CHAPTER 1 General provisions

    1. Article 1.. . . . . . . . . ....

    2. Article 2.. . . . . . . . . ....

  3. CHAPTER II Rules for the movement of equidae

    1. Article 3.. . . . . . . . . ....

    2. Article 4.. . . . . . . . . ....

    3. Article 5.. . . . . . . . . ....

    4. Article 6.. . . . . . . . . ....

    5. Article 7.. . . . . . . . . ....

    6. Article 8.. . . . . . . . . ....

    7. Article 9.. . . . . . . . . ....

    8. Article 10.. . . . . . . . . ....

  4. CHAPTER III Rules for imports from third countries

    1. Article 11.. . . . . . . . . ....

    2. Article 12.. . . . . . . . . ....

    3. Article 13.. . . . . . . . . ....

    4. Article 14.. . . . . . . . . ....

    5. Article 15.. . . . . . . . . ....

    6. Article 16.. . . . . . . . . ....

    7. Article 17.. . . . . . . . . ....

    8. Article 18.. . . . . . . . . ....

    9. Article 19.. . . . . . . . . ....

    10. Article 20.. . . . . . . . . ....

    11. Article 21.. . . . . . . . . ....

  5. CHAPTER IV Final provisions

    1. Article 22.. . . . . . . . . ....

    2. Article 23.. . . . . . . . . ....

    3. Article 24.. . . . . . . . . ....

    4. Article 25.. . . . . . . . . ....

    5. Article 26.. . . . . . . . . ....

    6. Article 27.. . . . . . . . . ....

    7. Article 28.. . . . . . . . . ....

    1. ANNEX A

      COMPULSORILY NOTIFIABLE DISEASES

      1. . . . . . . . . . ....

      2. . . . . . . . . . ....

    2. ANNEX B

      1. . . . . . . . . . ....

    3. ANNEX C

      1. . . . . . . . . . ....

      2. . . . . . . . . . ....

    4. ANNEX D

      AFRICAN HORSE SICKNESS

      1. . . . . . . . . . ....

      2. 1. COMPETITIVE ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...

        1. 1.1. Test procedure

          1. 1.1.1. Preparation of plates

            1. 1.1.1.1. . . . . . . . . . ....

            2. 1.1.1.2. . . . . . . . . . ....

          2. 1.1.2. Control wells

            1. 1.1.2.1. . . . . . . . . . ....

            2. 1.1.2.2. . . . . . . . . . ....

            3. 1.1.2.3. . . . . . . . . . ....

            4. 1.1.2.4. . . . . . . . . . ....

          3. 1.1.3. Spot test method

            1. 1.1.3.1. . . . . . . . . . ....

          4. 1.1.4. Serum titration method

            1. 1.1.4.1. . . . . . . . . . ....

          5. 1.1.5. Add 50 μl of guinea pig antisera, pre-diluted in blocking buffer,...

            1. 1.1.5.1. . . . . . . . . . ....

            2. 1.1.5.2. . . . . . . . . . ....

            3. 1.1.5.3. . . . . . . . . . ....

            4. 1.1.5.4. . . . . . . . . . ....

            5. 1.1.5.5. . . . . . . . . . ....

          6. 1.1.6. Chromogen

          7. 1.1.7. Reading

        2. 1.2. Expression of results

          1. 1.2.1. Using a software package print out the optical density (OD)...

          2. 1.2.2. The diagnostic threshold (cut-off value) for test sera is 50 %...

      3. 2. INDIRECT ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...

        1. 2.1. Test procedure

          1. 2.1.1. Solid phase

            1. 2.1.1.1. . . . . . . . . . ....

            2. 2.1.1.2. . . . . . . . . . ....

            3. 2.1.1.3. . . . . . . . . . ....

            4. 2.1.1.4. . . . . . . . . . ....

          2. 2.1.2. Test samples

            1. 2.1.2.1. Serum samples to be tested, and positive and negative control...

            2. 2.1.2.2. . . . . . . . . . ....

          3. 2.1.3. Conjugate

            1. 2.1.3.1. . . . . . . . . . ....

            2. 2.1.3.2. . . . . . . . . . ....

          4. 2.1.4. Cromogen/Substrate

            1. 2.1.4.1. Add 200 μl/well of chromogen/substrate solution (10 ml of 80,6 mM DMAB (dimethyl...

            2. 2.1.4.2. . . . . . . . . . ....

        2. 2.2. Interpretation of the results

          1. 2.2.1. . . . . . . . . . ....

          2. 2.2.2. . . . . . . . . . ....

          3. 2.2.3. . . . . . . . . . ....

          4. 2.2.4. . . . . . . . . . ....

      4. 3. BLOCKING ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...

        1. 3.1. Test procedure

          1. 3.1.1. ELISA plates

            1. 3.1.1.1. . . . . . . . . . ....

            2. 3.1.1.2. . . . . . . . . . ....

            3. 3.1.1.3. . . . . . . . . . ....

          2. 3.1.2. Test samples and controls

            1. 3.1.2.1. . . . . . . . . . ....

            2. 3.1.2.2. . . . . . . . . . ....

          3. 3.1.3. Conjugate

          4. 3.1.4. . . . . . . . . . ....

          5. 3.1.5. Chromogen/Substrate

          6. 3.1.6. Reading

        2. 3.2. Interpretation of the results

          1. 3.2.1. Assay validation

          2. 3.2.2. Cut-off calculation

          3. 3.2.3. Interpretation of results