Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC) (c. 432)

[F1 [F2 [F3 ANNEX C U.K. BRUCELLOSIS

Textual Amendments

F1 Substituted by Council Directive 97/12/EC of 17 March 1997 amending and updating Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine.

F2 Substituted by Commission Regulation (EC) No 535/2002 of 21 March 2002 amending Annex C to Council Directive 64/432/EEC and amending Decision 2000/330/EC.

F3 Substituted by Commission Decision of 10 December 2008 amending Annex C to Council Directive 64/432/EEC and Decision 2004/226/EC as regards diagnostic tests for bovine brucellosis (notified under document number C(2008) 7642) (Text with EEA relevance) (2008/984/EC).

2. IMMUNOLOGICAL TESTS U.K.

2.1. Standards U.K.

2.1.1. The Brucella abortus biovar 1 Weybridge strain No 99 or USDA strain 1119-3 must be used for the preparation of all antigens used in the rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and the milk ring test (MRT). U.K.

2.1.2. The standard reference serum for the RBT, SAT, CFT and MRT shall be the OIE international reference standard serum (OIEISS) formerly named WHO second international anti- Brucella abortus Serum (ISAbS). U.K.

2.1.3. The standard reference sera for enzyme-linked immunosorbent assays (ELISAs) shall be: U.K.

• the OIEISS,

• the weak positive OIE ELISA standard serum (OIEELISA _WP SS),

• the strong positive OIE ELISA standard serum (OIEELISA _SP SS),

• the negative OIE ELISA standard serum (OIEELISA _N SS).

2.1.4. The standard reference sera for fluorescence polarisation assays (FPAs) shall be: U.K.

• the weak positive OIE ELISA standard serum (OIEELISA _WP SS),

• the strong positive OIE ELISA standard serum (OIEELISA _SP SS),

• the negative OIE ELISA standard serum (OIEELISA _N SS).

2.1.5. The standard sera listed in 2.1.3 and 2.1.4 are available from the Community reference laboratory for brucellosis or the Veterinary Laboratories Agency (VLA), Weybridge, United Kingdom. U.K.

2.1.6. The OIEISS, the OIEELISA _WP SS, the OIEELISA _SP SS and the OIEELISA _N SS are international primary standards from which secondary reference national standards serum (working standards) must be established for each test referred to in 2.1.1 in each Member State.] ] ] U.K.