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The Animal By-Products (Wales) Regulations 2006

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Subcultured colonies

9.  After incubation each plate must be examined for colonies characteristic of Clostridium perfringens. All colonies characteristic of Clostridium perfringens must be–

(a)stab inoculated into motility nitrate medium(1); and

(b)inoculated into either lactose gelatin medium(2) or charcoal gelatin discs(3);

and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

(1)

Motility nitrate medium – See Hauschild AHW, Gilbert RJ, Harmon SM, O"Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892. National Research Council of Canada, Ottawa ON K1A oR6, Canada

(2)

Lactose gelatin medium – See Hauschild AHW, Gilbert RJ, Harmon SM, O"Keefe MF, Vahlefield R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892.

(3)

Charcoal gelatin discs – See Mackie and McCartney, (1996) Practical Medical Microbiology 14, 509. Churchill Livingstone, Robert Stevenson House, 1-3 Baxter"s Place, Leith Walk, Edinburgh EH1 3AF.

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