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SCHEDULE 3Testing Methods

PART IMETHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS

Samples with colonies of Clostridium perfringens

7.  If the surface area of the plates is overgrown and it is not possible to select well isolated characteristic colonies, 10 suspect colonies must be subcultured on to duplicate SF agar plates and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.