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SCHEDULE 3Testing Methods

PART IMETHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS

Inoculations

4.  Once the agar has set, each agar plate must be overlaid with a further 10 ml SF agar at a temperature of 47°C±1°C. Once the overlay has set and with the plate lids uppermost the plates must be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.