Regulation 16
1.—(1) The centre veterinarian must make a record of a bovine animal admitted that—
(a)did not belong to a herd officially free of enzootic bovine leukosis in accordance with Directive 64/432/EEC; or
(b)was produced by a dam which did not, after removal of the bovine animal from it, test negative to a test carried out in accordance with Annex D (Chapter II) to Directive 64/432/EEC.
(2) The centre veterinarian must pass a copy of any record made under sub-paragraph (1) to the centre veterinarian of any collection centre to which the bovine animals or their semen may move, not later than the date of such move.
2. The centre veterinarian must ensure that each individual dose of semen collected at the centre is clearly marked in such a way that the following information can be readily established—
(a)the date the semen was collected;
(b)the identity of the donor bovine animal under the cattle identification regulations;
(c)the breed of the donor bovine animal; and
(d)the licence number of the centre.
1.—(1) The centre veterinarian must ensure that all bovine animals at the centre are subjected at least once a year to the following tests—
(a)for bovine tuberculosis, an intradermal tuberculin test, carried out in accordance with the procedure laid down in Annex B to Directive 64/432/EEC;
(b)for bovine brucellosis, a serological test carried out in accordance with the procedure described in Annex C to Directive 64/432/EEC;
(c)for enzootic bovine leukosis, a serological test carried out in accordance with the procedure described in Annex D (Chapter II) to Directive 64/432/EEC;
(d)for IBR/IPV, a serological test (whole virus) on a blood sample; and
(e)for BVD/MD, a serological antibody test applied only to seronegative bovine animals;
(2) The centre veterinarian must ensure that bovine animals from which semen is being collected, or bovine animals having contact with such bovine animals, are subjected at least once a year to tests on samples of preputial specimen for—
(a)Campylobacter fetus ssp. venerealis; and
(b)Trichomonas foetus.
(3) The centre veterinarian must ensure that bovine animals from which semen is to be collected after an interval of more than six months are tested not more than 30 days prior to collection resuming on samples of preputial specimen for—
(a)Campylobacter fetus ssp. venerealis; and
(b)Trichomonas foetus.
(4) Should a bovine animal become serologically positive for BVD/MD, the centre veterinarian must ensure that every ejaculate of that bovine animal collected since the last negative test and until the date of the positive test is either discarded or used only if tested for the virus with negative results.
2.—(1) If any of the tests required under paragraph 1 is positive, the centre veterinarian must ensure that the bovine animal is isolated and that semen collected from it since the last negative test is (subject to paragraph 3) not supplied for intra-Community trade.
(2) The centre veterinarian must ensure that semen collected from all other bovine animals at the centre since the date when the positive test was carried out must be held in separate storage and may not be the subject of intra-Community trade until the health status of the centre has been restored to the level required by the Directive and these Regulations.
3. Notwithstanding paragraph 2(1), in the case of a bovine animal which has tested positive for BVD/MD under a serological antibody test, the centre veterinarian may allow semen from an ejaculate which has tested negative for the BVD/MD to be the subject of intra-Community trade.
1. The centre veterinarian must ensure that—
(a)semen processed at the centre is either—
(i)semen collected at an EC collection centre;
(ii)semen collected at a collection centre approved under the Directive in another part of the United Kingdom; or
(iii)semen which is from bovine animals which have been subjected to the tests specified in Schedule 2, paragraph 2(1);
(b)any semen referred to at sub-paragraph (a)(iii) is—
(i)processed using separate equipment (which must be cleaned and sterilised after use) or at a different time from the processing of semen intended for intra-Community trade; and
(ii)identified by a marking different from that required under sub-paragraph (f);
(c)semen collected, processed and quarantined at EC collection centres and intended for intra-Community trade does not come into contact with and is not stored in the same room as any other semen processed at the centre;
(d)products of animal origin used in the processing of semen, including additives and diluents, are obtained from sources which present no animal health risk or are so treated prior to use that such risk is removed;
(e)the cryogenic agent used has not been previously used for other products of animal origin;
(f)each individual dose of semen is sealed, numbered and clearly marked so that the following information can be readily established—
(i)the date the semen was collected;
(ii)the identity of the donor bovine animal under the cattle identification regulations;
(iii)the breed of the donor bovine animal; and
(iv)the licence number of the centre where the semen was collected (if applicable); and
(g)the format used for identifying semen is notified to the Secretary of State.
2. The centre veterinarian may not admit semen not collected at the centre for processing unless it is accompanied by—
(a)the documents specified in regulation 14(4); and
(b)if the semen comes from unlicensed premises, the documents specified in Schedule 7, paragraph 1(c).
3.—(1) Subject to sub-paragraph (2), the centre veterinarian must ensure that the antibiotics streptomycin, penicillin, lincomycin and spectinomycin are added to produce the following concentrations in the final diluted semen—
(a)not less than 500 μg streptomycin per ml final dilution,
(b)not less than 500 International Units penicillin per ml final dilution,
(c)not less than 150 μg lincomycin per ml final dilution, and
(d)not less than 300 μg spectinomycin per ml final dilution.
(2) An alternative combination of antibiotics which has an equivalent effect against campylobacters, leptospires and mycoplasmas may be used.
4. The centre veterinarian must ensure that, immediately after the addition of antibiotics, the semen is kept at a temperature of at least 5ºC for a period of not less than 45 minutes.
1.—(1) Prior to the initial dispatch of semen from bovine animals identified as serologically positive for BVD/MD, the centre veterinarian must ensure that a semen sample from each bovine animal is subjected to a virus isolation or virus antigen ELISA test for the BVD/MD virus.
(2) In the event of a positive result, the centre veterinarian must ensure that the bovine animal is removed from the collection centre and its semen destroyed.
2. The centre veterinarian must ensure that frozen semen is kept in the semen quarantine facilities for at least 30 days before it leaves the centre.
3. The centre veterinarian must not allow any semen to leave the centre unless the premises where it was collected remain clear of—
(a)foot and mouth disease; and
(b)the bovine diseases listed in Annex E(I) to Directive 64/432/EEC
for 30 days after collection or, in the case of fresh semen, until the date of dispatch of the semen.
4. With regard to a bovine animal that—
(a)did not belong to a herd officially free of enzootic bovine leukosis in accordance with Directive 64/432/EEC; or
(b)was produced by a dam which did not, after removal of the bovine animal from it, test negative to a test carried out in accordance with Annex D (Chapter II) to Directive 64/432/EEC,
the centre veterinarian must not allow its semen to leave the centre until the bovine animal has reached the age of two years and has tested negative for enzootic bovine leukosis, under a serological test carried out in accordance with the procedure described in Annex D (Chapter II) to Directive 64/432/EEC.
5. With regard to a bovine animal vaccinated against foot-and-mouth more than 30 days before, but within 12 months of, a collection, the centre veterinarian must not allow its semen to leave the centre unless 5% (with a minimum of five straws) of each collection have tested negative to a virus isolation test for foot-and-mouth disease.