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The Control of Substances Hazardous to Health Regulations 2002

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PART IIICONTAINMENT MEASURES FOR INDUSTRIAL PROCESSES

Containment measuresContainment levels
234
1Viable micro-organisms should be contained in a system which physically separates the process from the environment (closed system).YesYesYes
2Exhaust gases from the closed system should be treated so as to—Minimise releasePrevent releasePrevent release
3Sample collection, addition of materials to a closed system and transfer of viable micro-organisms to another closed system, should be performed so as to—Minimise releasePrevent releasePrevent release
4Bulk culture fluids should not be removed from the closed system unless the viable micro-organisms have been—Inactivated by validated meansInactivated by validated chemical or physical meansInactivated by validated chemical or physical means
5Seals should be designed so as to—Minimise releasePrevent releasePrevent release
6Closed systems should be located within a controlled area—OptionalOptionalYes, and purpose-built

(a)biohazard signs should be posted;

OptionalYesYes

(b)access should be restricted to nominated personnel only;

OptionalYesYes, via air-lock

(c)personnel should wear protective clothing;

Yes, work clothingYesYes, a complete change

(d)decontamination and washing facilities should be provided for personnel;

YesYesYes

(e)personnel should shower before leaving the controlled area;

NoOptionalYes

(f)effluent from sinks and showers should be collected and inactivated before release;

NoOptionalYes

(g)the controlled area should be adequately ventilated to minimise air contamination;

OptionalOptionalYes

(h)the controlled area should be maintained at an air pressure negative to atmosphere;

NoOptionalYes

(i)input and extract air to the controlled area should be HEPA filtered;

NoOptionalYes

(j)the controlled area should be designed to contain spillage of the entire contents of closed system;

OptionalYesYes

(k)the controlled area should be sealable to permit fumigation.

NoOptionalYes
7Effluent treatment before final discharge.Inactivated by validated meansInactivated by validated chemical or physical meansInactivated by validated physical means

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