34. The description of the proposed deliberate release, including the initial purpose or purposes of the release and any intention to use the genetically modified organism as or in a product in the future.
35. The intended dates of the release and time planning of the experiment including frequency and duration of releases.
36. The preparation of the site before the release.
37. The size of the site.
38. The method or methods to be used for the release.
39. The quantity of organisms to be released.
40. The disturbance on the site, including the type and method of cultivation, and mining, irrigation or other activities.
41. The worker protection measures taken during the release.
42. The post-release treatment of the site.
43. The techniques foreseen for elimination or inactivation of the organisms at the end of the experiment or other purpose of the release.
44. Information on, and the results of, previous releases of the organisms, and in particular, releases on a different scale or into different ecosystems.
45. The geographical location and national grid reference of the site or sites on to which the release will be made, or the foreseen areas of use of the product.
46. The physical or biological proximity of the site of the organisms to humans and other significant biota.
47. The proximity to significant biotopes, protected areas or drinking water supplies.
48. The climatic characteristics of the region or regions likely to be affected.
49. The geographical, geological and pedological characteristics.
50. The flora and fauna, including crops, livestock and migratory species.
51. The description of the target and non-target ecosystems likely to be affected.
52. The comparison of the natural habitat of the recipient organisms with the proposed site or sites of release.
53. Any known planned developments or changes in land use in the region which could influence the environmental impact of the release.