The Dairy Products (Hygiene) Regulations 1995

5.  In relation to the following tests, the methods for the analysis and testing shall be those specified below—

Test	Method
(a) Enumeration of Staphylococcus aureus	Provisional IDF Standard 145: 1990; Milk and milk-based products. Enumeration of Staphylococcus aureus in products other than dried milk-Colony count technique at 37°C.
(b) Enumeration of Stapjylococcus aureus in powdered milk	Provisional IDF Standard 138: 1986; Dried Milk. Enumeration of Staphylococcus aureus. Colony count technique at 37°C.
(c) Detection of Salmonella spp	ISO 6597: 1993; Microbiology— General guidance on methods for the detection of Salmonella and IDF Standard 93A: 1985; sample preparation.
(d) Detection of Listeria monocytogenes	ISO 10560: 1993; Milk and milk productgs—Detection of Listeria monocytogenes.
(e) Enumeration of Escherichia coli in milk-based products	Provisional IDF standard 170: 1994. Milk and milk products—Enumeration of presumptive Escherichia coli content by: (i) Most probable number technique. (ii) most probable number technique with use of 4-methylumbelliferyl –β –D–glucuronide (MUG). (iii) Colony count technique at 44°C using membranes.
(f) Detection of phosphatase (Residual) in cream	AOAC official method 950.41: Official Methods of Analysis, 16th edition: 1995.
(g) Detection of phosphatase (Reactivated and Residual) in cream	AOAC official method 965.27: Official Methods of Analysis, 16th edition: 1995.