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4. Quarter-strength Ringer’s solution shall be used. The composition of full-strength Ringer’s solution shall be —
Sodium chloride | 9.00 grams |
Potassium chloride | 0.42 grams |
Anhydrous calcium chloride | 0.24 grams |
Sodium bicarbonate | 0.20 grams |
Distilled water | 1,000 ml. |
Add 1 part of the above solution to 3 parts of distilled water. Fill into test tubes or bottles and sterilise by autoclaving at 121°deg. for 15 minutes. The quantity to be filled into the container before sterilisation must be predetermined to allow for evaporation losses during sterilisation.
Alternatively, sterile tubes and bottles may be filled aseptically with measured quantities of sterile quarter-strength Ringer’s solution.
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