Day 3
4. The RV broth shall be plated out on to two 90 millimetre plates of Brilliant Green Agar (BGA)(1), or on to one 90 millimetre plate of BGA and one 90 millimetre plate of Xylose Lysine Deoxycholate Agar (XLD)(2), using a 2.5 mm diameter loop. The plates shall be inoculated with a droplet taken from the edge of the surface of the fluid by drawing the loop over the whole of one plate in a zig zag pattern and continuing to the second plate without recharging the loop. The space between the loop streaks shall be 0.5 cm – 1.0 cm. The plates shall be incubated at 37°C±2°C for 24 hours ± 3 hours.
Brilliant Green Agar – see Edel W and Kampelmacher E H (1969) Bulletin of World Health Organisation 41:297–306, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042-9686).
Xylose Lisene Deoxycholate Agar – see Taylor W I, (1965) American Journal of Clinical Pathology, 44:471–475, Lippincott and Raven, 227E Washington Street, Philadelphia PA 19106, USA.