Day 2
3. The incubated BPW/L/G shall be added to Selenite Cystine Trimethylamine-N-Oxide Dulcitol (SC/T/D)(1) and Lysine Decarboxylase Glucose (LD/G)(2) media in electrical conductance cells or wells. For cells or wells containing more than 5 ml medium 0.2 ml of the BPW/L/G shall be added and for cells or wells containing 5 ml or less medium 0.1 ml of the BPW/L/G shall be added. Cells or wells shall be connected to appropriate electrical conductance measuring equipment set to monitor and record changes in electrical conductance at 6 minute intervals over a 24 hour period. The temperature of cells and wells shall be kept at 37°C.
Selenite Cystine Trimethylamine-N-Oxide Dulcitol – see Easter, M C and Gibson, D M, (1985) Journal of Hygiene 94:245–262, Cambridge University Press, Cambridge.
Lysine Decarboxylase Glucose – see Ogden I D (1988) International Journal of Food Microbiology 7:287–297, Elsevier Science BV, PO Box 211, 1000 AE, Amsterdam, Netherlands (ISSN 0168-1695).