Inoculations
3. For each portion of the sample 1 ml of solution shall be transferred to a sterile 90 mm petri dish (in duplicate), to which 15 ml of Shahidi – Ferguson agar (SF agar)(1) at a temperature of 47°C±1°C shall be added and immediately gently mixed by swirling the dish with 5 clockwise and 5 anticlockwise circular movements.
(1)
Shahidi-Ferguson agar – see Shahidi, S. A. and Ferguson, A. R. (1971) Applied Microbiology 21:500–506.American Society for Microbiology, 1913 1 St N.W., Washington DC 20006, USA.