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Regulation 16 and Schedule 4 Part I, paragraph 30

SCHEDULE 8CONTROL OF CERTAIN PROTEIN SOURCES

Column 1Column 2Column 3Column 4Column 5aColumn 6Column 7a
Name of product groupPermitted productsDesignation of nutritive principle or identity of micro-organismsCulture substrate (specifications, if any)Composition characteristics of productAnimal speciesName of product and specified particulars
a

In this Schedule the contents laid down or to be declared in accordance with Columns 5 and 7 refer to the product as such.

1.  Proteins obtained from the following groups of micro-organisms

1.1.  Bacteria

1.1.1.  Bacteria cultivated on methanol

1.1.1.1.  Protein product of fermentation obtained by culture of Methylophilus methylotrophus on methanol

Methylophilus methylotrophus NCIMB strain 10.515MethanolProtein: min 68% - Reflectance index: at least 50Pigs, calves, poultry and fish

Declarations to be made on the label or packaging of the product:

  • name of the product;

  • protein;

  • ash;

  • fat;

  • moisture content;

  • instructions for use;

  • “avoid inhalation”;

  • As from 1st April 2001: approval number;

Declarations to be made on the label or packaging of compound feeding stuffs:

—amount of the product contained in the feeding stuff

1.1.2.  Bacteria cultivated on natural gas

1.1.2.1  Protein product of fermentation from natural gas obtained by culture of:

Methylococcus capsulatus (Bath), Alcaligenes acidovorans, Bacillus brevis et Bacillus firmus, and the cells of which have been killed

Methyloccus capsulatus (Bath) NCIMB strain 11132 Alcaligenes acidovorans NCIMB strain 12387

Bacillus brevis

NCIMB strain 13288

Bacillus firmus

NCIMB strain 13280

Natural gas: (approx 91% methane, 5% ethane, 2% propane, 0.5% isobutane, 0.5% n butane, 1% other components), ammonia, mineral saltsProtein: min 65%
  • Pigs for fattening from 25 to 60kg

  • Calves from 80kg on

  • Salmon

Declarations to be made on the label or the packaging of the product:

  • the name “Protein product of fermentation from natural gas obtained by culture of Methylococcus capsulatus (Bath), Alcaligenes acidovorans, Bacillus brevis and Bacillus firmus

  • protein

  • ash

  • fat

  • moisture content

  • instructions for use

Maximum incorporation rate in the feed:

  • 8% pigs for fattening

  • 8% calves

  • 19% salmon (freshwater)

  • 33% salmon (seawater)

  • “avoid inhalation”;

Declarations to be made on the label or packaging of the compound feedingstuffs.

  • The name “Protein product obtained by bacterial fermentation of natural gas”

  • amount of the product contained in the feedingstuff

  • As from 1st April 2001: approval number

1.2.  Yeasts

1.2.1.  Yeasts cultivated on substrates of animal or vegetable ori

—Yeasts obtained from the micro-organisms and substrates listed in columns 3 and 4, the cells of which have been killed

Saccharomyces cerevisiae

Saccharomyces carlsbergiensis

Kluyveromyces lactis

Kluyveromyces fragilis

Molasses, distillery residues, cereals and products containing starch, fruit juice, whey, lactic acid, hydrolized vegetable fibresAll animal species

1.2.2.  Yeasts cultivated on substrates other than those given in 1.2.1

1.3.  Algae

1.4.  Lower fungi

1.4.1.  Products from production of antibiotics by fermentation

1.4.1.1.  Mycelium, wet by-product from the production of penicillin, ensiled by means of Lactobacillus brevis, plantarum, sake, collenoid and Streptococcus lactis to inactivate the penicillin, and heat treated

Nitrogenous compound Penicillium chrysogenum ATCC 48271Different sources of hydrolsatesNitrogen expressed as protein: min. 7%

Ruminants

Pigs

Declaration to be made on the label or packaging of the product:

  • the name: “Mycelium silage from the production of penicillin”;

  • Nitrogen expressed as protein;

  • ash;

  • moisture;

  • animal species or category;

  • As from 1st April 2001: approval number;

Declaration to be made on the label or packaging of the compound feeding stuff: the name: “mycellium silage from the production of penicillin”;

2.  Non-protein nitrogenous compound

2.1  Urea and its derivativ

2.1.1.  Urea, technically pure

2.1.2  Biuret, technically pure

2.1.3.  Urea-phosphate, technically pure

2.1.4.  Diureidoisobutane technically pure

CO(NH2)2

(CONH2)2 – NH

CO(NH2)2 H3PO4

(CH3)2 – (CH)2(NHCONH2)2

Urea min. 97%

Biuret: min. 97%

Nitrogen: min. 16.5%

Phosphorus: min. 18%

Nitrogen: min. 30%

Isobutyraldehyde: min. 35%

Ruminants from the beginning of rumination

Declarations to be made on the label or packaging of the product:

  • the name: “Urea”, “Biuret”, “Urea-phosphate” or “Diureidoisobutane”, as the case may be;

  • Nitrogen level; and in addition for product 2.1.3., phosphorus level;

  • animal species or category

Declarations to be made on the label or packaging of compound feeding stuffs:

  • the name “Urea”, “Biuret”, “Urea-phosphate” or “Diureidoisobutane”, as the case may be;

  • amount of the product contained in the feeding stuff;

  • percentage of the total protein provided by non-protein nitrogen;

  • indication, in the instructions for use, of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species or category

2.2.  Ammonium salts

2.2.1.  Ammonium lactate, produced by fermentation with Lactobacillus bulgaricus

CH3CHOHCOONH4WheyNitrogen expressed as protein: min. 44%Ruminants from the beginning of rumination

Declarations to be made on the label or packaging of the product:

  • the name: “Ammonium lactate from fermentation”;

  • nitrogen expressed as protein;

  • ash;

  • moisture;

  • animal species or category;

Declarations to be made on the label or packaging of compound feeding stuffs:

  • the name: “Ammonium lactate from fermentation”;

  • amount of product contained in the feeding stuff;

  • percentage of the total protein provided by non-protein nitrogen;

  • indication, in the instructions for use, of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species or category

2.2.2.  Ammonium acetate in aqueous solution

CH3COONH4Ammonium acetate: min 55%Ruminants from the start of rumination

Declarations to be made on the label or packaging of the product:

  • the words “Ammonium acetate”;

  • nitrogen content;

  • moisture content;

  • animal species or category;

Declarations to be made on the label or packaging of compound feeding stuffs:

  • the words “Ammonium acetate”;

  • the amount of the product contained in the feeding stuff;

  • percentage of the total protein provided by non-protein nitrogen;

  • indication in the instructions for use of the level of total non-protein nitrogen which should not be exceeded in the daily ration for each animal species or category

2.2.3.  Ammonium sulphate in aqueous solution

(NH4)2SO4Ammonium sulphate: min. 35%Ruminants, from the start of rumination

Declarations to be made on the label or packaging of the product:

  • the words “Ammonium sulphate”;

  • nitrogen and moisture contents;

  • animal species;

  • in the case of young ruminants, the incorporation rate in the daily ration may not exceed 0.5%;

Declaration to be made on the label or packaging of compound feeding stuffs:

  • the words “Ammonium sulphate”;

  • the amount of the product contained in the feeding stuff;

  • percentage of the total protein provided by non-protein nitrogen;

  • indication in the instructions for use of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species;

  • in the case of young ruminants, the incorporation rate in the daily ration may not exceed 0.5%

2.3.  By-products from the production of amino acids by fermentati

2.3.1.  Concentrated liquid by-products from the production of L-glutamic acid by fermentation with Corynebacterium melassecola

2.3.2.  Concentrated liquid by-products from the production of L-lysine monohydro-chloride by fermentation with Brevibacterium lactofermentum

Ammonium salts and other nitrogenous compounds

Ammonium salts and other Nitrogenous compounds

Sucrose, molasses, starch products and their hydrolysates

Sucrose, molasses, starch products and their hydrolysates

Nitrogen expressed as protein: min. 48%

Moisture max. 28%

Nitrogen expressed as protein: min 45%

Ruminants from the beginning of rumination

Ruminants from the beginning of rumination

Declarations to be made on the label or packaging of the product:

—the name “by products from the production of L-glutamic acid” in the case of product 2.3.1.;

  • “by-products from the production of L-lysine” in the case of product 2.3.2.;”; nitrogen, expressed as

  • protein

  • ash;

  • moisture;

  • animal species or category;

  • As from 1st April 2001: approval number;

Declarations to be made on the label or packaging of compound feeding stuffs:

  • percentage of the total protein provided by non-protein nitrogen;

  • indication, in the instructions for use, of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species or category

3.  Amino acids and their salts

All animal species

3.1.  Methionine

3.1.1.  DL-methionine, technically pure

3.1.2  Dihydrated calcium salt of N-hydroxymethyl-DL-methionine, technically pure

CH3S(CH2)2-CH(NH2)-COOH

[CH3 S(CH2)2- CH(NH-CH2OH)- COO]2CA.2H2O

DL-methionine: min 98%

DL-methionine: min 67%

Formaldehyde: max 14%

Calcium: min. 9%

Ruminants from the beginning of rumination

Declarations to be made on the label or packaging of the product:

—the name: “DL-methionine”, in the case of product 3.1.1.

3.1.3.  Methionine-zinc, technically pure

[CH3S(CH2)2-CH(NH2)-COO]2Zn

DL-methionine: min 80%

Zn: max 18.5%

  • DL-methionine and moisture content;

  • animal species or category in the case or products 3.1.2., and 3.1.3.;

  • As from 1st April 2001: approval number;

3.1.4.  Concentrated liquid sodium

DL-methionine technically pure

(CH3S(CH2)2-CH(NH2)-COO)Na

DL-methionine: min 40%

Sodium: min. 6.2%

All animal species

Declarations to be made on the label or packaging of the product:

  • the name: “concentrated liquid sodium DL-methionine”;

  • DL-methionine content:

  • moisture content;

  • As from 1st April 2001: approval number;

3.1.5.  DL-methionine, technically pure protected with copolymer vinylpyridine/styrene

CH3S(CH2)2-CH(NH2)-COOHDL-methionine: min 65% copolymer vinylpyridine/ styrene: max 3%Dairy cows

Declarations to be made on the label or packaging of the product:

  • “Protected methionine with copolymer vinylpyridine/ styrene”;

  • DL-methionine and moisture contents;

  • animal species;

  • As from 1st April 2001: approval number;

3.2.  Lysine

3.2.1.  L-Lysine, technically pure

3.2.2.  Concentrated liquid L-Lysine (base)

3.2.3.  L-lysine-monohydrochloride, technically pure

3.2.4.  Concentrated liquid L-Lysine-monohydrochloride

3.2.5.  L-Lysine sulphate produced by fermentation with Corynebacterium glutamicum

NH2 – (CH2)4-CH(NH2)-COOH

NH2(CH2)4-CH(NH2)-COOH

NH2-(CH2)4-CH(NH2)-COOH.HC1

[NH2(CH2)4-CH(NH2)-COOH.HC1

[NH2(CH2)4-CH(NH2)-COOH]2-H2SO4

Saccharose, molasses, starch products and their hydrolysates

Saccharose, molasses, starch products and their hydrolysates

Sugar syrup, molasses, cereals, starch products and their hydrolysates

L-Lysine: min 98%

L-Lysine: min 50%

L-Lysine: min 78%

L-Lysine: min 22.4%

L-Lysine: min 40%

All animal species

Declarations to be made on the label or packaging of the product:

—the name “L-Lysine” in the case of product 3.2.1., “Concentrated liquid L-Lysine base” in the case of product 3.2.2.,

“L-Lysine monohydrochloride” in the case of product 3.2.3.,

“Concentrated liquid L-Lysine monohydrochloride” in the case of product 3.2.4.

“L-Lysine sulphate and its by-products from fermentation” in the case of product 3.2.5.;

L-Lysine and moisture content;

—As from 1st April 2001: approval number;

3.2.6.  L-Lysine phosphate and its by-products produced by fermentation with Brevibacterium lactofermentatum NRRLB-11470

[NH2(CH2)4-CH(NH2)-COOH]-H3PO4Sucrose ammonia and fish solubles

L-Lysine: min 35%

Phosphorus: min 43%

Poultry

Pigs

Declarations to be made on the label or packaging of the product:

  • the name “L-Lysine phosphate and its by-products from fermentation”

  • L-Lysine and moisture content;

  • As from 1st April 2001: approval number;

3.2.7.  Mixtures of:

(a)L-Lysine-mono-hydrochloride, technically pure and

(b)DL-methionine technically pure protected with copolymer vinyl-pyridine/styrene

NH2-(CH2)4-CH(NH2)-COOH-HC1

CH3S(CH2)-2-CH(NH2)-COOH

L-Lysine + DL-methionine: minimum 50% (including DL-methionine: minimum 15%)

Copolymer vinyl pyridine/ styrene: maximum 3%

Dairy cows

Declarations to be made on the label or packaging of the product:

  • the name “mixture of L-Lysine monohydrochloride and DL-methionine protected with copolymer vinyl-pyridine/styrene”;

  • L-Lysine, DL-methionine and moisture contents;

  • animal species;

  • As from 1st April 2001: approval number;

3.3.  Threonine

3.3.1.  L-Threonine, technically pure

CH3CH(OH)-CH(NH2) – COOHL-Threonine: min 98%All animal species

Declarations to be made on the label or packaging of the product:

  • the name “L-Threonine”

  • L-Threonine and moisture content;

  • As from 1st April 2001: approval number;

3.4.  Tryptopha

3.4.1.  L-Tryptophan, technically pure

(C8H5NH)-CH2-CH(NH2)-COOHL-Tryptophan: min. 98%All animal species

Declarations to be made on the label or packaging of the product:

  • the name: “L-Tryptophan”;

  • L-Tryptophan and moisture content;

  • As from 1st April 2001: approval number;

3.4.2.  DL-Tryptophan, technically pure

(C8H5NH)-CH2-CH(NH2)-COOHDL-Tryptophan: min. 98%All animal species

Declarations to be made on the label or packaging of the product:

  • the name “DL-Tryptophan”;

  • DL Tryptophan and moisture content;

  • As from 1st April 2001: approval number;

4.  Analogues of amino acids

4.1.  Analogues of methionine

4.1.1.  Hydroxy analogue of methionine

4.1.2  Calcium salt of hydroxy analogue of methionine

CH3S(CH2)2-CH(OH)-COOH

[CH3S-(CH2)-CH(OH)-COO]2Ca

Total of acids: minimum 85%

Monomer acid minimum 65%

Monomer acid: Minimum 83%

Calcium: minimum 12%

All animal species

Declarations to be made on the label or packaging of the product:

  • if appropriate, the name (column 2);

  • monomer acid and total acids contents in the case of product 4.1.1. and monomer acid content in the case of product 4.1.2.;

  • moisture content;

  • animal species;

  • As from 1st April 2001: approval number;

Declarations to be made on the label or packaging of compound feeding stuffs:

  • if appropriate, the name (column 2);

  • monomer acid and total acids contents in the case of product 4.1.1. and monomer acid content in the case of product 4.1.2.;

  • amount of the product contained in the feeding stuff.