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SCHEDULE 3Sampling and Testing Methods

Part IIMethod for the Isolation of clostridium perfringens

Inoculations

3.  For each portion of the sample 1 ml of suspension/solution shall be transferred to a sterile 90 mm petri dish (in duplicate), to which 15 ml of egg yolk-free tryptose sulphite cycloserine agar (EY-free TSC agar)(1) at a temperature of 46°C ± 1°C shall be added and immediately gently mixed by swirling the dish with 5 clockwise and 5 anticlockwise circular movements.

(1)

EY- free TSC agar. See Hauschild, A.H.W. and Hilsheimer, R. (1974) Applied Microbiology 27:78–82. American Society for Microbiology, 1913 1 St N.W., Washington DC 20006, USA Back [1]