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3A. When chemical checks are to be carried out by the district council samples must be taken and subjected to laboratory analysis for the control of the following parameters—
(a)TVB-N (Total Volatile Basic-Nitrogen), in respect of which—
(i)the following TVB-N limits must not be exceeded—
25 milligrams of nitrogen per 100 grams of flesh for the following species:
Sebastes spp.;
Helicolenus dactylopterus;
Sebastichthys capensis;
30 milligrams of nitrogen per 100 grams of flesh for the following species
all species belonging to the Pleuronectidae family (with the exception of halibut: Hippoglossus spp.);
35 milligrams of nitrogen per 100 grams of flesh for the following species
Salmo salar;
species belonging to the Merlucciidae family;
species belonging to the Gadidae family;
(ii)the reference method to be used for checking the TVB-N limit is the method involving distillation of an extract deproteinised by perchloric acid as set out in Annexes II and III of Commission Decision 95/149/EC of 8th March 1995(1) (“the Decision”) read together with article 3 of the Decision;
(iii)the routine methods which may be used to check the TVB-N limit are those specified in article 2(3) of the Decision;
(iv)the sample must consist of about 100 grams of flesh, taken from at least 3 different points and mixed together by grinding;
(b)TMA-N (Trimethylamine-Nitrogen);
(c)Histamine, in respect of which—
(i)nine samples must be taken from each batch; these must fulfil the following requirements—
the mean value must not exceed 100 parts per million (“ppm”);
two samples may have a value of more than 100 ppm but less than 200 ppm;
no sample may have a value exceeding 200 ppm;
(ii)these limits apply only to fish species of the following families: Scombridae, Clupeidae, Engraulidae and Coryphaenidae; however, fish belonging to these families which have undergone enzyme ripening treatment in brine may have higher histamine levels but not more than twice the above values; examinations must be carried out in accordance with reliable, scientifically recognised methods, such as high-performance liquid chromatography (HPLC).
O.J. No. L97, 29.4.95, p. 84