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Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance)
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CHAPTER II RULES FOR THE MOVEMENT OF EQUIDAE BETWEEN MEMBER STATES
Article 3.Member States shall authorise the movement of registered equidae in...
Article 4.(1) Equidae must show no clinical sign of disease at...
Article 5.(1) A Member State which is not free from African...
Article 6.Member States which implement an alternative control system providing guarantees...
Article 7.(1) Equidae must be transported, as soon as possible, from...
Article 8.(1) Member States shall ensure that: (a) registered equidae which...
Article 9.The rules laid down in Directive 90/425/EEC shall apply in...
Article 10.Veterinary experts from the Commission may, to the extent necessary...
CHAPTER III RULES FOR IMPORTATION OF EQUIDAE FROM THIRD COUNTRIES
Article 11.Equidae imported into the Community must satisfy the conditions laid...
Article 12.(1) The importation of equidae into the Community shall only...
Article 13.(1) The equidae must come from third countries which:
Article 14.Before the day of loading for transportation to the Member...
Article 15.Importation of equidae from the territory of a third country...
Article 16.(1) The equidae must be identified in accordance with Article...
Article 17.(1) Immediately upon arrival in the Member State of destination,...
Article 18.Checks shall be carried out on the spot by veterinary...
Article 19.In accordance with the procedure referred to in Article 21(2):...
Article 20.Annexes I to IV shall be amended in accordance with...
Article 21.(1) The Commission shall be assisted by the Standing Committee...
Article 22.Directive 90/426/EEC, as amended by the acts listed in Annex...
Article 23.This Directive shall enter into force on the 20th day...
Article 24.This Directive is addressed to the Member States.
1. Indirect ELISA for the detection of antibodies to African horse...
1.1.1.1. Coat ELISA plates with recombinant AHSV-4 VP7 diluted in carbonate/bicarbonate...
1.1.1.2. Wash the plates five times with distilled water containing 0,01...
1.1.1.3. Block the plates with phosphate buffered saline (PBS) pH 7,2...
1.1.1.4. Remove the blocking solution and gently tap the plates onto...
2. Blocking ELISA for the detection of antibodies to African horse...
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