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[F1ANNEX VIU.K. METHODS OF ANALYSIS FOR THE DETERMINATION OF CONSTITUENTS OF ANIMAL ORIGIN FOR THE OFFICIAL CONTROL OF FEED

2. METHODS U.K.

2.1. Light microscopy U.K.

2.1.2. Reagents and equipment U.K.
2.1.2.2. Equipment U.K.
2.1.2.2.1. Analytical balance with an accuracy of 0,001 g U.K.
2.1.2.2.2. [F2Grinding equipment: knife or rotor mill. If a rotor mill is used, mill sieves ≤ 0,5 mm shall be prohibited] U.K.
2.1.2.2.3. [F2Sieves with square meshes of 0,25 mm and 1 mm width. With the exception of sample pre-sieving, the diameter of the sieves should not exceed 10 cm to avoid loss of materials. Calibration of sieves is not required] U.K.
2.1.2.2.4.Conical glass separation funnel with a content of 250 ml with Teflon or ground glass stopcock at the base of the cone. Stopcock opening diameter shall be ≥ 4mm. Alternatively, a conical bottomed settling beaker may be used provided the laboratory has demonstrated that detection levels are equivalent to that obtained using the conical glass separation funnel.U.K.
Separation funnel U.K.
2.1.2.2.5.Stereomicroscope covering at least a 6,5× to 40× final magnification rangeU.K.
2.1.2.2.6.Compound microscope covering at least a 100× to 400× final magnification range with transmitted light bright field. Polarised light and differential interferential contrast can additionally be usedU.K.
2.1.2.2.7. Standard laboratory glassware U.K.
2.1.2.2.8. Equipment for slide preparation: classical microscope slides, hollow slides, coverslips (20 × 20 mm), tweezers, fine spatula U.K.
[F32.1.2.2.9. Laboratory oven U.K.
2.1.2.2.10. Centrifuge U.K.
2.1.2.2.11. Filter paper: qualitative cellulose filter (pore size 4-11 μm)]] U.K.