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Directive 2010/63/EU of the European Parliament and of the CouncilShow full title

Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (Text with EEA relevance)

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1.Mice, rats, gerbils, hamsters and guinea pigs

In this and subsequent tables for mice, rats, gerbils, hamsters and guinea pigs, ‘enclosure height’ means the vertical distance between the enclosure floor and the top of the enclosure and this height applies over more than 50 % of the minimum enclosure floor area prior to the addition of enrichment devices.

When designing procedures, consideration shall be given to the potential growth of the animals to ensure adequate space is provided (as detailed in Tables 1.1 to 1.5) for the duration of the study.

Table 1.1. Mice
a

Post-weaned mice may be kept at these higher stocking densities for the short period after weaning until issue, provided that the animals are housed in larger enclosures with adequate enrichment, and these housing conditions do not cause any welfare deficit such as increased levels of aggression, morbidity or mortality, stereotypes and other behavioural deficits, weight loss, or other physiological or behavioural stress responses.

Body weight(g)Minimum enclosure size(cm2)Floor area per animal(cm2)Minimum enclosure height(cm)Date referred to in Article 33(2)
In stock and during proceduresup to 2033060121 January 2017
over 20 to 253307012
over 25 to 303308012
over 3033010012
Breeding

330

For a monogamous pair (outbred/inbred) or a trio (inbred). For each additional female plus litter 180 cm2 shall be added.

12
Stock at breedersaEnclosure size950 cm2less than 209504012
Enclosure size1 500 cm2less than 201 5003012
Table 1.2. Rats
a

In long-term studies, if space allowances per individual animal fall below those indicated above towards the end of such studies, priority shall be given to maintaining stable social structures.

b

Post-weaned rats may be kept at these higher stocking densities for the short period after weaning until issue, provided that the animals are housed in larger enclosures with adequate enrichment, and these housing conditions do not cause any welfare deficit such as increased levels of aggression, morbidity or mortality, stereotypes and other behavioural deficits, weight loss, or other physiological or behavioural stress responses.

Body weight(g)Minimum enclosure size(cm2)Floor area per animal(cm2)Minimum enclosure height(cm)Date referred to in Article 33(2)
In stock and during proceduresaup to 200800200181 January 2017
over 200 to 30080025018
over 300 to 40080035018
over 400 to 60080045018
over 6001 50060018
Breeding

800

Mother and litter. For each additional adult animal permanently added to the enclosure add 400 cm2

18
Stock at breedersbEnclosure size1 500 cm2up to 501 50010018
over 50 to 1001 50012518
over 100 to 1501 50015018
over 150 to 2001 50017518
Stock at breedersbEnclosure size2 500 cm2up to 1002 50010018
over 100 to 1502 50012518
over 150 to 2002 50015018
Table 1.3. Gerbils
Body weight(g)Minimum enclosure size(cm2)Floor area per animal(cm2)Minimum enclosure height(cm)Date referred to in Article 33(2)
In stock and during proceduresup to 401 200150181 January 2017
over 401 20025018
Breeding

1 200

Monogamous pair or trio with offspring

18
Table 1.4. Hamsters
a

Post-weaned hamsters may be kept at these higher stocking densities, for the short period after weaning until issue provided that the animals are housed in larger enclosures with adequate enrichment, and these housing conditions do not cause any welfare deficit such as increased levels of aggression, morbidity or mortality, stereotypes and other behavioural deficits, weight loss, or other physiological or behavioural stress responses.

Body weight(g)Minimum enclosure size(cm2)Floor area per animal(cm2)Minimum enclosure height(cm)Date referred to in Article 33(2)
In stock and during proceduresup to 60800150141 January 2017
over 60 to 10080020014
over 10080025014
Breeding

800

Mother or monogamous pair with litter

14
Stock at breedersaless than 601 50010014
Table 1.5. Guinea pigs
Body weight(g)Minimum enclosure size(cm2)Floor area per animal(cm2)Minimumenclosure height(cm)Date referred to in Article 33(2)
In stock and during proceduresup to 2001 800200231 January 2017
over 200 to 3001 80035023
over 300 to 4501 80050023
over 450 to 7002 50070023
over 7002 50090023
Breeding

2 500

Pair with litter. For each additional breeding female add 1 000 cm2

23

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