Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance) (c. 156)

[F1 ANNEX IV U.K. AFRICAN HORSE SICKNESS DIAGNOSIS

Textual Amendments

F1 Substituted by Commission Implementing Decision (EU) 2016/1840 of 14 October 2016 amending Annex IV to Council Directive 2009/156/EC as regards methods for African horse sickness diagnosis (notified under document C(2016) 6509) (Text with EEA relevance).

PART A U.K. Serological tests

2. Blocking ELISA for the detection of antibodies to African horse sickness virus (AHSV) U.K.

2.1. Test procedure U.K.

2.1.1. Solid Phase U.K.

2.1.1.1. Coat ELISA plates with 50-100 ng of recombinant AHSV-4 VP7 diluted in carbonate/bicarbonate buffer, pH 9,6. Incubate overnight at 4 °C. U.K.

2.1.1.2. Wash the plates three times with phosphate buffered saline (PBS) 0,1× containing 0,135 M NaCl and 0,05 % (v/v) Tween 20 (PBST). Gently tap the plates on to absorbent material to remove any residual wash.] U.K.