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This buffer is used for extraction of the bacterium from plant tissues by homogenisation or shaking.
Na2HPO4 (anhydrous) | 4,26 g |
KH2PO4 | 2,72 g |
Distilled water | 1.00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.
Additional components may be useful as follows:
This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.
Na2HPO4.12H2O | 2,7 g |
NaH2PO4.2H2O | 0,4 g |
Distilled water | 1,00 L |
Dissolve ingredients, check pH and sterilise by autoclaving at 121 °C for 15 min.