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[F1ANNEX II U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF RALSTONIA SOLANACEARUM (SMITH) YABUUCHI ET AL.

SECTION VI U.K. OPTIMISED PROTOCOLS FOR DETECTION AND IDENTIFICATION OF R. SOLANACEARUM

B. IDENTIFICATION TESTS U.K.

2. IF test U.K.
2.1. Prepare a suspension of approximately 10 6 cells per ml in IF buffer (Appendix 4). U.K.
2.2. Prepare a twofold dilution series of an appropriate antiserum (see website http://forum.europa.eu.int/Public/irc/sanco/Home/main). U.K.
2.3. Apply the IF procedure (Section VI.A.5.). U.K.
2.4. A positive IF test is achieved if the IF titre of the culture is equivalent to that of the positive control.] U.K.